C57BL/6JCya-Ro60em1/Cya
Common Name:
Ro60-KO
Product ID:
S-KO-19454
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Ro60-KO
Strain ID
KOCMP-20822-Ro60-B6J-VC
Gene Name
Product ID
S-KO-19454
Gene Alias
1810007I17Rik; A530054J02Rik; SS-A/Ro; Ssa; Ssa2; Trove2
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ro60em1/Cya mice (Catalog S-KO-19454) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000159879
NCBI RefSeq
NM_013835
Target Region
Exon 3~5
Size of Effective Region
~2.1 kb
Detailed Document
Overview of Gene Research
Ro60, also known as SSA2, is an RNA-binding protein and a core component of nucleocytoplasmic ribonucleoprotein (RNP) complexes. It is essential in RNA metabolism, cell stress response pathways, and maintaining cellular homeostasis. Ro60 stabilizes and mediates quality control and cellular distribution of small RNAs like YRNAs, retroelement transcripts, and misfolded RNAs [1].
Knockdown of Ro60/SSA by siRNA transfection significantly decreased cell proliferation and invasion in vitro, and inhibited the growth of subcutaneous tumors in vivo, indicating its role in promoting the progression of pancreatic ductal adenocarcinoma [2]. In addition, Ro60 deletion in human cell lines led to enhanced expression of Alu RNAs and interferon-regulated genes, establishing a link among Ro60, Alu retroelements, and type I interferon [3].
In conclusion, Ro60 is crucial for RNA-related functions and cellular homeostasis. Model-based research, such as the siRNA-mediated knockdown in pancreatic cancer and gene deletion in cell lines, reveals its significance in cancer progression and autoimmune-related inflammation. Understanding Ro60's function provides insights into the molecular mechanisms of autoimmune diseases and cancer, potentially offering new therapeutic targets.
References：
1. Mahla, Ranjeet Singh, Jones, Esther L, Dustin, Lynn B. 2024. Ro60-Roles in RNA Processing, Inflammation, and Rheumatic Autoimmune Diseases. In International journal of molecular sciences, 25, . doi:10.3390/ijms25147705. https://pubmed.ncbi.nlm.nih.gov/39062948/
2. Liu, Dan, Qian, Wenbiao, Li, Deliang, Kong, Lingjian. 2017. Ro60/SSA levels are increased and promote the progression of pancreatic ductal adenocarcinoma. In Biochemical and biophysical research communications, 495, 2519-2524. doi:10.1016/j.bbrc.2017.12.124. https://pubmed.ncbi.nlm.nih.gov/29274781/
3. Hung, T, Pratt, G A, Sundararaman, B, Yeo, G W, Behrens, T W. 2015. The Ro60 autoantigen binds endogenous retroelements and regulates inflammatory gene expression. In Science (New York, N.Y.), 350, 455-9. doi:10.1126/science.aac7442. https://pubmed.ncbi.nlm.nih.gov/26382853/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen