C57BL/6JCya-Faap20em1/Cya
Common Name:
Faap20-KO
Product ID:
S-KO-19509
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Faap20-KO
Strain ID
KOCMP-67513-Faap20-B6J-VC
Gene Name
Product ID
S-KO-19509
Gene Alias
2610002J02Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
4
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Faap20em1/Cya mice (Catalog S-KO-19509) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000178473
NCBI RefSeq
NM_001190445
Target Region
Exon 2~3
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
Faap20, also known as 20-kDa FANCA-associated protein, is an integral component of the Fanconi anemia (FA) core complex. It plays essential roles in DNA repair pathways, especially in homology-directed repair of DNA double-strand breaks, which is crucial for maintaining genome integrity [1]. The FA pathway, in which Faap20 is involved, is a multi-step DNA repair process at stalled replication forks in response to DNA interstrand cross-links (ICLs) [2].
Deletion of Faap20 in mice led to a mild FA-like phenotype with defects in the reproductive and hematopoietic systems. Faap20 -/- mice are susceptible to mitomycin C (MMC)-induced pancytopenia. Faap20 -/- hematopoietic stem and progenitor cells (HSPCs) showed defects in long-term multilineage reconstitution and aberrant cell cycle patterns during chronic MMC treatment [4].
In conclusion, Faap20 is vital for DNA repair and genome maintenance, as demonstrated by the phenotypes in Faap20 knockout mouse models. These models help us understand the role of Faap20 in the context of diseases related to FA, such as hematologic defects and cancer predisposition [3,4].
References：
1. Palovcak, Anna, Yuan, Fenghua, Verdun, Ramiro, Luo, Liang, Zhang, Yanbin. 2023. Fanconi anemia associated protein 20 (FAAP20) plays an essential role in homology-directed repair of DNA double-strand breaks. In Communications biology, 6, 873. doi:10.1038/s42003-023-05252-9. https://pubmed.ncbi.nlm.nih.gov/37620397/
2. Wang, Jingming, Chan, Bryan, Tong, Michael, Haley, John, Kim, Hyungjin. 2019. Prolyl isomerization of FAAP20 catalyzed by PIN1 regulates the Fanconi anemia pathway. In PLoS genetics, 15, e1007983. doi:10.1371/journal.pgen.1007983. https://pubmed.ncbi.nlm.nih.gov/30789902/
3. Ali, Abdullah Mahmood, Pradhan, Arun, Singh, Thiyam Ramsingh, Pang, Qishen, Meetei, Amom Ruhikanta. 2012. FAAP20: a novel ubiquitin-binding FA nuclear core-complex protein required for functional integrity of the FA-BRCA DNA repair pathway. In Blood, 119, 3285-94. doi:10.1182/blood-2011-10-385963. https://pubmed.ncbi.nlm.nih.gov/22343915/
4. Zhang, Tingting, Wilson, Andrew F, Mahmood Ali, Abdullah, Ruhikanta Meetei, Amom, Pang, Qishen. 2015. Loss of Faap20 Causes Hematopoietic Stem and Progenitor Cell Depletion in Mice Under Genotoxic Stress. In Stem cells (Dayton, Ohio), 33, 2320-30. doi:10.1002/stem.2048. https://pubmed.ncbi.nlm.nih.gov/25917546/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen