C57BL/6JCya-Cdr2em1/Cya
Common Name:
Cdr2-KO
Product ID:
S-KO-19516
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Cdr2-KO
Strain ID
KOCMP-12585-Cdr2-B6J-VB
Gene Name
Product ID
S-KO-19516
Gene Alias
-
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cdr2em1/Cya mice (Catalog S-KO-19516) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000033169
NCBI RefSeq
NM_007672
Target Region
Exon 3~4
Size of Effective Region
~1.8 kb
Detailed Document
Overview of Gene Research
Cdr2, or cerebellar degeneration-related protein 2, is an onconeural antigen. In fission yeast cells, the kinase Cdr2 forms membrane-bound nodes during interphase, which are crucial for controlling the localization of mitotic signaling proteins and the cytokinetic ring, and also play a role in timely cell cycle progression and cell size control [2,3,4,5]. In mammalian cells, it may be involved in regulating ER sheet organization as an adaptor for cytoplasmic dynein-1 [6].
In paraneoplastic cerebellar degeneration (PCD), anti-Yo autoantibodies often target Cdr2 and its paralogue Cdr2L. In a study on PCA-1/anti-Yo paraneoplastic autoimmunity, CDR2 testing alone may produce false-positive results, and optimal performance was achieved by requiring both CDR2 and CDR2L positivity in serum and CDR2L positivity in CSF [1]. Another study found that CDR2 localizes to the nucleus and co-localizes with nuclear speckle proteins, while Yo antibodies specifically bind to CDR2L in Purkinje neurons of PCD patients, potentially interfering with ribosomal machinery function [7].
In conclusion, Cdr2 has diverse functions in cell cycle and size control in fission yeast and may be implicated in the pathogenesis of paraneoplastic cerebellar degeneration. Studies on Cdr2, especially in the context of its role in PCD, help in understanding the disease mechanisms and may contribute to the development of better diagnostic and therapeutic strategies for this autoimmune-related neurological disorder.
References:
1. Vorasoot, Nisa, Scharf, Madeleine, Miske, Ramona, Ott, Anthonina, McKeon, Andrew. 2023. CDR2 and CDR2L line blot performance in PCA-1/anti-Yo paraneoplastic autoimmunity. In Frontiers in immunology, 14, 1265797. doi:10.3389/fimmu.2023.1265797. https://pubmed.ncbi.nlm.nih.gov/37841252/
2. Opalko, Hannah, Geng, Shuhan, Hall, Aaron R, Vavylonis, Dimitrios, Moseley, James B. 2023. Design principles of Cdr2 node patterns in fission yeast cells. In Molecular biology of the cell, 34, br18. doi:10.1091/mbc.E23-04-0135. https://pubmed.ncbi.nlm.nih.gov/37610834/
3. Facchetti, Giuseppe, Knapp, Benjamin, Flor-Parra, Ignacio, Chang, Fred, Howard, Martin. 2019. Reprogramming Cdr2-Dependent Geometry-Based Cell Size Control in Fission Yeast. In Current biology : CB, 29, 350-358.e4. doi:10.1016/j.cub.2018.12.017. https://pubmed.ncbi.nlm.nih.gov/30639107/
4. Opalko, Hannah, Geng, Shuhan, Hall, Aaron R, Vavylonis, Dimitrios, Moseley, James B. 2023. Design principles of Cdr2 node patterns in fission yeast cells. In bioRxiv : the preprint server for biology, , . doi:10.1101/2023.04.19.537536. https://pubmed.ncbi.nlm.nih.gov/37131752/
5. Opalko, Hannah E, Miller, Kristi E, Kim, Hyun-Soo, Keogh, Michael-Christopher, Moseley, James B. 2021. Arf6 anchors Cdr2 nodes at the cell cortex to control cell size at division. In The Journal of cell biology, 221, . doi:10.1083/jcb.202109152. https://pubmed.ncbi.nlm.nih.gov/34958661/
6. Teixeira, Vanessa, Singh, Kashish, Gama, José B, Carter, Andrew P, Gassmann, Reto. 2024. CDR2 is a dynein adaptor recruited by kinectin to regulate ER sheet organization. In bioRxiv : the preprint server for biology, , . doi:10.1101/2024.11.06.622207. https://pubmed.ncbi.nlm.nih.gov/39574738/
7. Herdlevaer, Ida, Kråkenes, Torbjørn, Schubert, Manja, Vedeler, Christian A. 2020. Localization of CDR2L and CDR2 in paraneoplastic cerebellar degeneration. In Annals of clinical and translational neurology, 7, 2231-2242. doi:10.1002/acn3.51212. https://pubmed.ncbi.nlm.nih.gov/33009713/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen