C57BL/6JCya-Cpsf1em1/Cya
Common Name:
Cpsf1-KO
Product ID:
S-KO-19801
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Cpsf1-KO
Strain ID
KOCMP-94230-Cpsf1-B6J-VB
Gene Name
Product ID
S-KO-19801
Gene Alias
CPSF160
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
15
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cpsf1em1/Cya mice (Catalog S-KO-19801) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000230157
NCBI RefSeq
NM_001164173
Target Region
Exon 2~25
Size of Effective Region
~5.6 kb
Detailed Document
Overview of Gene Research
Cpsf1, also known as cleavage and polyadenylation specificity factor 1, is a critical factor involved in alternative polyadenylation (APA), a common RNA modification process. It plays a role in the 3' untranslated region processing of a subset of pre-mRNAs, which is essential for normal gene expression regulation and various biological processes [5]. It is associated with multiple pathways such as the MAPK/ERK pathway [1].
In various disease models, Cpsf1 shows diverse functions. In a diabetic retinopathy model, down-regulation of Cpsf1 was observed in diabetic tissues and high-glucose-induced human retinal vascular endothelial cells. Up-regulation of Cpsf1 alleviated apoptosis and migration via the MAPK/ERK pathway [1]. In Caenorhabditis elegans, circ-CPSF1 (a circular RNA related to Cpsf1) was found to have deleterious effects on lifespan, eggs production and germ cell apoptosis through oxidative stress after ionizing radiation [2]. In gastric cancer, Cpsf1 is up-regulated, and its depletion weakened cell proliferation and metastasis. Cpsf1 positively regulates NSDHL by APA to promote cancer progression [3]. In prostate cancer, Cpsf1 is up-regulated in advanced cases, and its knockdown inhibited cell growth, reduced glycolytic output, and led to widespread use of intergenic poly(A) sites [4].
In conclusion, Cpsf1 is essential for regulating gene expression through APA and is involved in multiple biological processes and disease conditions. Studies using different models, including disease-specific cell models and animal models like zebrafish and C. elegans, have revealed its role in diseases such as diabetic retinopathy, radiation-induced injury, and various cancers, providing insights into potential therapeutic targets.
References:
1. Zhang, Jingyi, Zhang, Xi, Zou, Yuanyuan, Han, Fengmei. 2020. CPSF1 mediates retinal vascular dysfunction in diabetes mellitus via the MAPK/ERK pathway. In Archives of physiology and biochemistry, 128, 708-715. doi:10.1080/13813455.2020.1722704. https://pubmed.ncbi.nlm.nih.gov/32046510/
2. Yuan, Jing, Lin, Fei, Wu, Zhiyong, Li, Li, Zhang, Chao. 2023. Circ-CPSF1 Worsens Radiation-Induced Oxidative Stress Injury in Caenorhabditis elegans. In Biomolecules, 13, . doi:10.3390/biom13010102. https://pubmed.ncbi.nlm.nih.gov/36671487/
3. Kang, Weibiao, Yang, Yang, Chen, Changyu, Yu, Changjun. 2022. CPSF1 positively regulates NSDHL by alternative polyadenylation and promotes gastric cancer progression. In American journal of cancer research, 12, 4566-4583. doi:. https://pubmed.ncbi.nlm.nih.gov/36381317/
4. Tietz, Kiel T, McCluskey, Braedan M, Miller, Conor R, Munro, Sarah A, Dehm, Scott M. 2025. CPSF1 inhibition promotes widespread use of intergenic polyadenylation sites and impairs glycolysis in prostate cancer cells. In Cell reports, 44, 115211. doi:10.1016/j.celrep.2024.115211. https://pubmed.ncbi.nlm.nih.gov/39847481/
5. Bolli, Niccolò, Payne, Elspeth M, Rhodes, Jennifer, Zon, Leonard I, Look, A Thomas. 2011. cpsf1 is required for definitive HSC survival in zebrafish. In Blood, 117, 3996-4007. doi:10.1182/blood-2010-08-304030. https://pubmed.ncbi.nlm.nih.gov/21330472/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen