C57BL/6JCya-Cyp7b1em1/Cya
Common Name:
Cyp7b1-KO
Product ID:
S-KO-19990
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Cyp7b1-KO
Strain ID
KOCMP-13123-Cyp7b1-B6J-VA
Gene Name
Product ID
S-KO-19990
Gene Alias
D3Ertd552e; hct-1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cyp7b1em1/Cya mice (Catalog S-KO-19990) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000035625
NCBI RefSeq
NM_007825
Target Region
Exon 2
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
CYP7B1, a cytochrome P450 enzyme, hydroxylates carbons 6 and 7 of the B ring of oxysterols and steroids. This action reduces substrate biological activity and aids their conversion into excretable end-products. It is involved in cholesterol metabolism, especially in inactivating oxysterols and converting them to bile salts in the liver [1].
In KO mouse models, CYP7B1 deficiency attenuates experimental autoimmune encephalomyelitis, reducing leukocyte infiltration, suppressing pathogenic CD4+ T cell proliferation, and decreasing myeloid cell activation in the central nervous system [2]. In Cyp7b1-/-mice, cold-induced BAT-mediated clearance of fatty acids from triglyceride-rich lipoproteins is reduced, along with compromised BAT function and reduced fecal bile acid levels [4]. BM-MSCs from CYP7B1 conditional knockout mice have impaired activation abilities, related to delayed bone defect healing [3].
In conclusion, CYP7B1 is crucial for cholesterol metabolism, with its deficiency influencing multiple biological processes. The study of CYP7B1 KO/CKO mouse models has provided insights into its role in diseases such as neuroinflammatory diseases, cold-induced lipoprotein clearance, and stem cell activation, highlighting its potential as a therapeutic target in these areas.
References:
1. Stiles, Ashlee R, McDonald, Jeffrey G, Bauman, David R, Russell, David W. 2009. CYP7B1: one cytochrome P450, two human genetic diseases, and multiple physiological functions. In The Journal of biological chemistry, 284, 28485-9. doi:10.1074/jbc.R109.042168. https://pubmed.ncbi.nlm.nih.gov/19687010/
2. Song, Huanhuan, Lv, Aowei, Zhu, Zhibao, Wang, Ning, Fu, Ying. 2024. CYP7B1 deficiency impairs myeloid cell activation in autoimmune disease of the central nervous system. In PNAS nexus, 3, pgae334. doi:10.1093/pnasnexus/pgae334. https://pubmed.ncbi.nlm.nih.gov/39262855/
3. Zhang, Zhaoqiang, Su, Zepeng, Li, Zhikun, Shen, Huiyong, Xie, Zhongyu. 2024. CYP7B1-mediated 25-hydroxycholesterol degradation maintains quiescence-activation balance and improves therapeutic potential of mesenchymal stem cells. In Cell chemical biology, 31, 1277-1289.e7. doi:10.1016/j.chembiol.2024.01.009. https://pubmed.ncbi.nlm.nih.gov/38382532/
4. Evangelakos, Ioannis, Kuhl, Anastasia, Baguhl, Miriam, Heeren, Joerg, Worthmann, Anna. 2022. Cold-Induced Lipoprotein Clearance in Cyp7b1-Deficient Mice. In Frontiers in cell and developmental biology, 10, 836741. doi:10.3389/fcell.2022.836741. https://pubmed.ncbi.nlm.nih.gov/35478959/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen