C57BL/6JCya-Clca2em1/Cya
Common Name:
Clca2-KO
Product ID:
S-KO-20035
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Clca2-KO
Strain ID
KOCMP-229933-Clca2-B6J-VB
Gene Name
Product ID
S-KO-20035
Gene Alias
4732440A06; Clca5
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Clca2em1/Cya mice (Catalog S-KO-20035) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000040465
NCBI RefSeq
NM_178697
Target Region
Exon 3
Size of Effective Region
~1.7 kb
Detailed Document
Overview of Gene Research
Clca2, short for Calcium-activated chloride channel A2, is a transmembrane protein with diverse functions. It has been implicated in multiple biological processes, often functioning as a tumor suppressor. It is associated with pathways like Wnt/β -catenin, ERK/JNK/p38-MAPK, and is involved in regulating calcium-activated chloride channel currents and store-operated calcium entry [1,2,5]. Genetic models, such as gene knockout models, can be valuable for further exploring its functions.
In cervical cancer, Clca2 expression is decreased compared to adjacent normal tissues. Its up-regulation is associated with longer survival times, and knockdown of Clca2 by small interfering RNA suppresses tumor cell proliferation and migration, suggesting it suppresses the proliferation, migration, and invasion of cervical cancer cells [1].
In breast cancer, CLCA2 is frequently inactivated by promoter region hypermethylation, and when overexpressed in CLCA2-negative cell lines, their tumorigenicity and metastasis capability are significantly reduced [7]. In African American women with triple negative breast cancer, CLCA2 expression is associated with overall survival [6].
Additionally, in keratinocytes, CLCA2 is transported to the nucleus via extracellular vesicles, where it binds to and activates β-catenin, affecting cell survival and migration [4]. Depletion of Clca2 in UVB and Nutlin3a-induced senescence models led to accelerated senescence onset, highlighting its role in cellular senescence and potentially skin aging [3].
In conclusion, Clca2 is a crucial protein with significant implications in multiple disease areas, especially cancer. Its functions in suppressing tumor cell behaviors in cervical and breast cancer, as well as its role in cellular senescence, have been revealed through various studies. Gene knockout models could potentially further clarify its functions in these and other biological processes and disease conditions.
References:
1. Zhang, Peijin, Lin, Yang, Liu, Yaqiong. 2021. CLCA2 suppresses the proliferation, migration and invasion of cervical cancer. In Experimental and therapeutic medicine, 22, 776. doi:10.3892/etm.2021.10208. https://pubmed.ncbi.nlm.nih.gov/34055075/
2. Xin, Wenhu, Zhang, Jian, Zhang, Haibin, Li, Yufeng, Wang, Fang. 2022. CLCA2 overexpression suppresses epithelial-to-mesenchymal transition in cervical cancer cells through inactivation of ERK/JNK/p38-MAPK signaling pathways. In BMC molecular and cell biology, 23, 44. doi:10.1186/s12860-022-00440-7. https://pubmed.ncbi.nlm.nih.gov/36280802/
3. Guerrero-Navarro, Lena, Martic, Ines, Ploner, Christian, Jansen-Dürr, Pidder, Cavinato, Maria. 2024. CLCA2: A Potential Guardian against Premature Senescence and Skin Aging. In Biomedicines, 12, . doi:10.3390/biomedicines12030592. https://pubmed.ncbi.nlm.nih.gov/38540205/
4. Seltmann, Kristin, Hettich, Britta, Abele, Seraina, Leroux, Jean-Christophe, Werner, Sabine. . Transport of CLCA2 to the nucleus by extracellular vesicles controls keratinocyte survival and migration. In Journal of extracellular vesicles, 13, e12430. doi:10.1002/jev2.12430. https://pubmed.ncbi.nlm.nih.gov/38602325/
5. Sharma, Aarushi, Ramena, Grace, Yin, Yufang, Premkumar, Louis, Elble, Randolph C. 2018. CLCA2 is a positive regulator of store-operated calcium entry and TMEM16A. In PloS one, 13, e0196512. doi:10.1371/journal.pone.0196512. https://pubmed.ncbi.nlm.nih.gov/29758025/
6. Purrington, Kristen S, Knight, Jimmie, Dyson, Gregory, Boerner, Julie L, Bandyopadhyay, Sudeshna. 2020. CLCA2 expression is associated with survival among African American women with triple negative breast cancer. In PloS one, 15, e0231712. doi:10.1371/journal.pone.0231712. https://pubmed.ncbi.nlm.nih.gov/32298355/
7. Li, Xiurong, Cowell, John K, Sossey-Alaoui, Khalid. . CLCA2 tumour suppressor gene in 1p31 is epigenetically regulated in breast cancer. In Oncogene, 23, 1474-80. doi:. https://pubmed.ncbi.nlm.nih.gov/14973555/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen