C57BL/6JCya-Mobpem1/Cya
Common Name:
Mobp-KO
Product ID:
S-KO-20071
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Mobp-KO
Strain ID
KOCMP-17433-Mobp-B6J-VB
Gene Name
Product ID
S-KO-20071
Gene Alias
MOBP155; MOBP170; MOBP69; MOBP73; MOBP81
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
9
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mobpem1/Cya mice (Catalog S-KO-20071) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000068698
NCBI RefSeq
NM_001039365
Target Region
Exon 3~4
Size of Effective Region
~3.9 kb
Detailed Document
Overview of Gene Research
Mobp, short for myelin-associated oligodendrocyte basic protein, is a relatively abundant CNS-specific myelin protein. It plays a crucial role in stabilizing the myelin sheath in the central nervous system (CNS), which is essential for the rapid saltatory conduction of action potentials within the CNS and for sustaining neuronal health [3]. The non-receptor tyrosine kinase Fyn regulates MOBP synthesis, and MOBP affects the morphological differentiation of oligodendrocytes [6].
Genetic studies suggest that a locus between RPSA and MOBP may contribute to an increased risk of sporadic frontotemporal dementia (sFTD) through effects on gene expression and/or splicing in the brain cortex [1]. In multiple system atrophy (MSA), MOBP mRNA levels decrease, correlated with increased DNA methylation, and MOBP mislocalizes into glial cytoplasmic inclusions where it interacts with α-synuclein, indicating its relevance to MSA pathogenesis [2]. In multiple sclerosis (MS), MOBP is considered a primary target autoantigen, with T-cell autoimmunity against it detectable in patients [3]. However, MOBP-specific cellular immune responses are weaker than MOG-specific ones in MS patients and healthy subjects [7]. In a Greek population, the rs616147 polymorphism of MOBP was not associated with amyotrophic lateral sclerosis (ALS) [4]. Neonatal antibiotic-induced gut dysbiosis led to increased expression of MOBP and other myelin-related genes in the pre-frontal cortex of mice, affecting the microbiota-gut-brain axis [5]. Inhibiting MOBP expression blocked the long-lasting antidepressant effects of ketamine, suggesting its role in myelination-related antidepressant mechanisms [8]. A genome-wide association study identified a region near MOBP associated with non-viral HCC in European descent populations from North America [9].
In conclusion, Mobp is essential for myelin sheath stability and oligodendrocyte morphological differentiation. Its dysregulation is implicated in various neurodegenerative diseases such as sFTD, MSA, and MS, as well as in non-neurological conditions like HCC. Functional studies, including those using potential genetic models, have provided insights into its role in these disease processes, helping to understand the underlying molecular mechanisms and potentially guiding future therapeutic strategies.
References:
1. Manzoni, Claudia, Kia, Demis A, Ferrari, Raffaele, Hardy, John, Escott-Price, Valentina. 2024. Genome-wide analyses reveal a potential role for the MAPT, MOBP, and APOE loci in sporadic frontotemporal dementia. In American journal of human genetics, 111, 1316-1329. doi:10.1016/j.ajhg.2024.05.017. https://pubmed.ncbi.nlm.nih.gov/38889728/
2. Bettencourt, Conceição, Miki, Yasuo, Piras, Ignazio S, Huentelman, Matt J, Holton, Janice L. 2021. MOBP and HIP1 in multiple system atrophy: New α-synuclein partners in glial cytoplasmic inclusions implicated in the disease pathogenesis. In Neuropathology and applied neurobiology, 47, 640-652. doi:10.1111/nan.12688. https://pubmed.ncbi.nlm.nih.gov/33368549/
3. Kaushansky, Nathali, Eisenstein, Miriam, Zilkha-Falb, Rina, Ben-Nun, Avraham. 2009. The myelin-associated oligodendrocytic basic protein (MOBP) as a relevant primary target autoantigen in multiple sclerosis. In Autoimmunity reviews, 9, 233-6. doi:10.1016/j.autrev.2009.08.002. https://pubmed.ncbi.nlm.nih.gov/19683076/
4. Liampas, Ioannis, Siokas, Vasileios, Aloizou, Athina-Maria, Hadjigeorgiou, Georgios M, Dardiotis, Efthimios. 2021. MOBP rs616147 Polymorphism and Risk of Amyotrophic Lateral Sclerosis in a Greek Population: A Case-Control Study. In Medicina (Kaunas, Lithuania), 57, . doi:10.3390/medicina57121337. https://pubmed.ncbi.nlm.nih.gov/34946282/
5. Keogh, Ciara E, Kim, Danielle H J, Pusceddu, Matteo M, Barboza, Mariana, Gareau, Mélanie G. 2020. Myelin as a regulator of development of the microbiota-gut-brain axis. In Brain, behavior, and immunity, 91, 437-450. doi:10.1016/j.bbi.2020.11.001. https://pubmed.ncbi.nlm.nih.gov/33157256/
6. Schäfer, Isabelle, Müller, Christina, Luhmann, Heiko J, White, Robin. 2016. MOBP levels are regulated by Fyn kinase and affect the morphological differentiation of oligodendrocytes. In Journal of cell science, 129, 930-42. doi:10.1242/jcs.172148. https://pubmed.ncbi.nlm.nih.gov/26801084/
7. Jilek, Samantha, Schluep, Myriam, Pantaleo, Giuseppe, Du Pasquier, Renaud A. 2012. MOBP-specific cellular immune responses are weaker than MOG-specific cellular immune responses in patients with multiple sclerosis and healthy subjects. In Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology, 34, 539-43. doi:10.1007/s10072-012-1144-4. https://pubmed.ncbi.nlm.nih.gov/22752855/
8. Huang, Chaoli, Wu, Zifeng, Wang, Di, Hashimoto, Kenji, Yang, Chun. 2023. Myelin-associated oligodendrocytic basic protein-dependent myelin repair confers the long-lasting antidepressant effect of ketamine. In Molecular psychiatry, 29, 1741-1753. doi:10.1038/s41380-023-02288-5. https://pubmed.ncbi.nlm.nih.gov/37848708/
9. Hassan, Manal M, Li, Donghui, Han, Younghun, Roberts, Lewis R, Amos, Christopher I. 2024. Genome-wide association study identifies high-impact susceptibility loci for HCC in North America. In Hepatology (Baltimore, Md.), 80, 87-101. doi:10.1097/HEP.0000000000000800. https://pubmed.ncbi.nlm.nih.gov/38381705/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen