C57BL/6JCya-Lacc1em1/Cya
Common Name:
Lacc1-KO
Product ID:
S-KO-20227
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Lacc1-KO
Strain ID
KOCMP-210808-Lacc1-B6J-VB
Gene Name
Product ID
S-KO-20227
Gene Alias
9030625A04Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
14
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Lacc1em1/Cya mice (Catalog S-KO-20227) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000062789
NCBI RefSeq
NM_172488
Target Region
Exon 4
Size of Effective Region
~0.8 kb
Detailed Document
Overview of Gene Research
Lacc1, also known as C13orf31, is an enzyme highly expressed in inflammatory macrophages. It converts L-citrulline to L-ornithine and isocyanic acid, bridging proinflammatory nitric oxide synthase (NOS2) and polyamine immunometabolism. This function is central to its regulatory role in multiple inflammatory diseases such as inflammatory bowel diseases, arthritis, and microbial infections [1,2].
In Lacc1 -/- mice, more severe colon lesions were observed after oral administration of Citrobacter rodentium, and accelerated onset of arthritis and more severe inflammation occurred in collagen-induced arthritis models. Also, Lacc1 -/- mice had increased bacteria burden in intestinal lymphoid organs, more severe T-cell transfer colitis, and altered T-cell cytokine profiles. In macrophages, Lacc1 was required for toll-like receptor-induced bacteria uptake, reactive oxygen and nitrogen species production, and autophagy [3,4]. In a mouse model of inflammatory bowel disease, Lacc1 deletion aggravated disease, as seen by higher disease activity index scores, faster weight loss, and shorter colon lengths [5].
In conclusion, Lacc1 is essential for maintaining the balance between antibacterial defenses and intestinal inflammation, as well as regulating autophagy and T-cell cytokine responses. Gene knockout mouse models have been crucial in revealing its role in diseases like inflammatory bowel disease, arthritis, and microbial infections, highlighting its potential as a therapeutic target for these inflammation-related conditions.
References:
1. Wei, Zheng, Oh, Joonseok, Flavell, Richard A, Crawford, Jason M. 2022. LACC1 bridges NOS2 and polyamine metabolism in inflammatory macrophages. In Nature, 609, 348-353. doi:10.1038/s41586-022-05111-3. https://pubmed.ncbi.nlm.nih.gov/35978195/
2. Li, Yaling, Wu, Zhixiong, Tan, Xiaoli, Tang, Liang, Ouyang, Fan. 2023. LACC1: A critical involvement in macrophage immunometabolism. In Cell biology international, 47, 1488-1490. doi:10.1002/cbin.12063. https://pubmed.ncbi.nlm.nih.gov/37366569/
3. Skon-Hegg, Cara, Zhang, Juan, Wu, Xiumin, Lee, Wyne P, Behrens, Timothy W. 2018. LACC1 Regulates TNF and IL-17 in Mouse Models of Arthritis and Inflammation. In Journal of immunology (Baltimore, Md. : 1950), 202, 183-193. doi:10.4049/jimmunol.1800636. https://pubmed.ncbi.nlm.nih.gov/30510070/
4. Kang, Jung-Woo, Yan, Jie, Ranjan, Kishu, Turner, Jerrold R, Abraham, Clara. 2020. Myeloid Cell Expression of LACC1 Is Required for Bacterial Clearance and Control of Intestinal Inflammation. In Gastroenterology, 159, 1051-1067. doi:10.1053/j.gastro.2020.07.024. https://pubmed.ncbi.nlm.nih.gov/32693188/
5. Xu, Zheng-Yuan, Wang, Jin-Chun. 2023. LACC1 regulates changes in the intestinal flora in a mouse model of inflammatory bowel disease. In BMC gastroenterology, 23, 358. doi:10.1186/s12876-023-02971-5. https://pubmed.ncbi.nlm.nih.gov/37848840/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen