C57BL/6JCya-Ccn4em1/Cya
Common Name:
Ccn4-KO
Product ID:
S-KO-20288
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Ccn4-KO
Strain ID
KOCMP-22402-Ccn4-B6J-VB
Gene Name
Product ID
S-KO-20288
Gene Alias
Elm1; Wisp1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
15
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ccn4em1/Cya mice (Catalog S-KO-20288) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000005255
NCBI RefSeq
NM_018865
Target Region
Exon 2
Size of Effective Region
~1.7 kb
Detailed Document
Overview of Gene Research
Ccn4, also known as WNT1-Inducible Signaling Pathway Protein 1 (WISP1), is a highly conserved, secreted cysteine-rich matricellular protein located on chromosome 8q24.1-8q24.3 [2]. It is involved in multiple cellular mechanisms such as cell proliferation, migration, invasion, angiogenesis, wound healing, repair, and apoptosis [1]. Ccn4 is a downstream target of β-catenin and has an intricate relationship with pathways like protein kinase B (Akt), mechanistic target of rapamycin (mTOR), AMP-activated protein kinase (AMPK), silent mating type information regulation 2 homolog 1 (Saccharomyces cerevisiae) (SIRT1), and mammalian forkhead transcription factors (FoxOs) [4]. It is of great biological importance as its aberrant expression is associated with numerous disease pathologies including cancer, fibrosis, metabolic and inflammatory disorders [1].
In a high-fat-fed ApoE-/-mouse model, Ccn4 overexpression led to reduced occlusion of the brachiocephalic artery with less apoptosis, fewer macrophages, and attenuated lipid core size, while Ccn4 deficiency resulted in increased apoptosis and occlusion as well as increased plaque in the aortic root [3]. In another study using apolipoprotein-E knockout (ApoE-/ -) mice fed a high-fat diet and infused with Angiotensin II, deletion of Ccn4 significantly reduced the number of ruptured aortae, aortic area, and aneurysm grade score, along with decreased macrophage infiltration and cell apoptosis [5].
In conclusion, Ccn4 plays essential roles in cell survival, migration, and apoptosis, which are crucial in various disease conditions. The use of gene knockout mouse models, such as ApoE-/-mice with Ccn4 deletion, has revealed its significant impact on atherosclerosis and aneurysm progression, providing potential therapeutic implications for these diseases.
References:
1. Singh, Kirti, Oladipupo, Sunday S. 2024. An overview of CCN4 (WISP1) role in human diseases. In Journal of translational medicine, 22, 601. doi:10.1186/s12967-024-05364-8. https://pubmed.ncbi.nlm.nih.gov/38937782/
2. Gaudreau, Pierre-Olivier, Cousineau, Isabelle, Stagg, John. . Optimal CCN4 Immunofluorescence for Tissue Microarray. In Methods in molecular biology (Clifton, N.J.), 2582, 13-21. doi:10.1007/978-1-0716-2744-0_2. https://pubmed.ncbi.nlm.nih.gov/36370340/
3. Williams, Helen, Simmonds, Steven, Bond, Andrew, Johnson, Jason, George, Sarah. 2024. CCN4 (WISP-1) reduces apoptosis and atherosclerotic plaque burden in an ApoE mouse model. In Atherosclerosis, 397, 118570. doi:10.1016/j.atherosclerosis.2024.118570. https://pubmed.ncbi.nlm.nih.gov/39276419/
4. Maiese, Kenneth. . Prospects and Perspectives for WISP1 (CCN4) in Diabetes Mellitus. In Current neurovascular research, 17, 327-331. doi:10.2174/1567202617666200327125257. https://pubmed.ncbi.nlm.nih.gov/32216738/
5. Williams, Helen, Wadey, Kerry S, Frankow, Aleksandra, Johnson, Jason L, George, Sarah J. 2021. Aneurysm severity is suppressed by deletion of CCN4. In Journal of cell communication and signaling, 15, 421-432. doi:10.1007/s12079-021-00623-5. https://pubmed.ncbi.nlm.nih.gov/34080128/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen