C57BL/6JCya-Slc1a1em1/Cya
Common Name:
Slc1a1-KO
Product ID:
S-KO-20322
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Slc1a1-KO
Strain ID
KOCMP-20510-Slc1a1-B6J-VB
Gene Name
Product ID
S-KO-20322
Gene Alias
D130048G10Rik; EAAC1; EAAC2; EAAT3; MEAAC1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
19
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Slc1a1em1/Cya mice (Catalog S-KO-20322) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000025875
NCBI RefSeq
NM_009199
Target Region
Exon 3~4
Size of Effective Region
~2.5 kb
Detailed Document
Overview of Gene Research
Slc1a1, also known as excitatory amino acid carrier 1 (EAAC1), is a sodium-dependent glutamate/cysteine transporter. It plays a central role in regulating neuronal glutathione (GSH) production, and is involved in processes like glutamate uptake, which limits excitotoxicity [4,5]. It also participates in cystine uptake and GSH biosynthesis in cancer cells, and is associated with various biological pathways related to cell metabolism and redox homeostasis [6].
In solid tumors, HIF-1α enhances the transcription of SLC1A1, promoting cystine uptake and ferroptosis resistance [1]. In IDH1-mutant solid tumors, SLC1A1 facilitates the influx of R-2-hydroxyglutarate into endothelial cells, promoting tumor angiogenesis [2]. In natural killer T-cell lymphoma, SLC1A1 promotes tumor growth by reprogramming glutamine metabolism, and its overexpression is associated with PD-L1 downregulation [3]. SLC1A1 deficiency in mice abolishes mIDH1-promoted tumor angiogenesis and the therapeutic benefit of mIDH1 inhibitor in solid tumors [2].
In conclusion, Slc1a1 is crucial for maintaining glutamate homeostasis, GSH production, and is involved in multiple disease-related processes. Mouse models with Slc1a1 deficiency have been instrumental in revealing its role in tumor angiogenesis and ferroptosis resistance in solid tumors, as well as its impact on lymphoma progression, thus providing insights into potential therapeutic strategies for these diseases.
References:
1. Yang, Zhou, Su, Wei, Wei, Xiyi, Zen, Ke, Yao, Bing. 2023. HIF-1α drives resistance to ferroptosis in solid tumors by promoting lactate production and activating SLC1A1. In Cell reports, 42, 112945. doi:10.1016/j.celrep.2023.112945. https://pubmed.ncbi.nlm.nih.gov/37542723/
2. Wang, Xiaomin, Chen, Ziqi, Xu, Jun, Li, Leping, Huang, Min. 2022. SLC1A1-mediated cellular and mitochondrial influx of R-2-hydroxyglutarate in vascular endothelial cells promotes tumor angiogenesis in IDH1-mutant solid tumors. In Cell research, 32, 638-658. doi:10.1038/s41422-022-00650-w. https://pubmed.ncbi.nlm.nih.gov/35459936/
3. Xiong, Jie, Wang, Nan, Zhong, Hui-Juan, Huang, Jin-Yan, Zhao, Wei-Li. 2021. SLC1A1 mediated glutamine addiction and contributed to natural killer T-cell lymphoma progression with immunotherapeutic potential. In EBioMedicine, 72, 103614. doi:10.1016/j.ebiom.2021.103614. https://pubmed.ncbi.nlm.nih.gov/34628354/
4. Aoyama, Koji. 2021. Glutathione in the Brain. In International journal of molecular sciences, 22, . doi:10.3390/ijms22095010. https://pubmed.ncbi.nlm.nih.gov/34065042/
5. Karan, Kalpita Rashimi, Satishchandra, P, Sinha, Sanjib, Anand, Anuranjan. 2017. Rare SLC1A1 variants in hot water epilepsy. In Human genetics, 136, 693-703. doi:10.1007/s00439-017-1778-7. https://pubmed.ncbi.nlm.nih.gov/28324217/
6. Guo, Wenzheng, Li, Kaimi, Sun, Beibei, Lin, Shu-Hai, Deng, Jiong. 2020. Dysregulated Glutamate Transporter SLC1A1 Propels Cystine Uptake via Xc- for Glutathione Synthesis in Lung Cancer. In Cancer research, 81, 552-566. doi:10.1158/0008-5472.CAN-20-0617. https://pubmed.ncbi.nlm.nih.gov/33229341/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen