C57BL/6JCya-Prrc2aem1/Cya
Common Name:
Prrc2a-KO
Product ID:
S-KO-20429
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Prrc2a-KO
Strain ID
KOCMP-53761-Prrc2a-B6J-VC
Gene Name
Product ID
S-KO-20429
Gene Alias
3110039B05Rik; Bat-2; Bat2; D17H6S51E; G2; Wbp12; mKIAA4178
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
17
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Prrc2aem1/Cya mice (Catalog S-KO-20429) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000025253
NCBI RefSeq
NM_020027
Target Region
Exon 12
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
Prrc2a, Proline rich coiled-coil 2A, is an m6A reader that plays crucial roles in multiple biological processes. It is involved in regulating mRNA metabolism and translation, and is associated with pathways related to cell differentiation, development, and disease [1,2,3]. Genetic models, especially gene knockout (KO) and conditional knockout (CKO) mouse models, have been instrumental in studying its functions.
Germ cell-specific knockout of Prrc2a in mice leads to XY asynapsis, impaired meiotic sex chromosome inactivation, and meiotic arrest at metaphase I during spermatogenesis, indicating its essential role in male fertility [1]. Nestin-Cre-mediated knockout in mice shows that Prrc2a controls oligodendrocyte specification and myelination, as its deletion causes hypomyelination, decreased lifespan, and locomotive and cognitive defects [2]. In the context of cancer, intestinal epithelium-specific deletion of Prrc2a suppresses tumor cell growth, stemness, and migratory capacity in colorectal cancer, while its overexpression promotes these behaviors [3].
In conclusion, Prrc2a is essential for meiosis I completion in spermatogenesis, oligodendroglial specification and myelination, and has a significant impact on cancer progression. KO/CKO mouse models have been key in revealing its role in these biological processes and disease conditions, providing valuable insights into potential therapeutic targets for male infertility, hypomyelination-related neurological diseases, and cancers such as colorectal cancer [1,2,3].
References:
1. Tan, Xinshui, Zheng, Caihong, Zhuang, Yinghua, Jin, Pengpeng, Wang, Fengchao. 2023. The m6A reader PRRC2A is essential for meiosis I completion during spermatogenesis. In Nature communications, 14, 1636. doi:10.1038/s41467-023-37252-y. https://pubmed.ncbi.nlm.nih.gov/36964127/
2. Wu, Rong, Li, Ang, Sun, Baofa, Yang, Yun-Gui, Yuan, Zengqiang. 2018. A novel m6A reader Prrc2a controls oligodendroglial specification and myelination. In Cell research, 29, 23-41. doi:10.1038/s41422-018-0113-8. https://pubmed.ncbi.nlm.nih.gov/30514900/
3. Wu, Xi, Wang, Shiyang, Pan, Yuwei, Yu, Lu, Yu, Zhengquan. 2024. m6A Reader PRRC2A Promotes Colorectal Cancer Progression via CK1ε-Mediated Activation of WNT and YAP Signaling Pathways. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 12, e2406935. doi:10.1002/advs.202406935. https://pubmed.ncbi.nlm.nih.gov/39582289/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen