C57BL/6JCya-Hebp2em1/Cya
Common Name
Hebp2-KO
Product ID
S-KO-20701
Backgroud
C57BL/6JCya
Strain ID
KOCMP-56016-Hebp2-B6J-VB
Status
When using this mouse strain in a publication, please cite “Hebp2-KO Mouse (Catalog S-KO-20701) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Hebp2-KO
Strain ID
KOCMP-56016-Hebp2-B6J-VB
Gene Name
Product ID
S-KO-20701
Gene Alias
SOUL
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 10
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000020000
NCBI RefSeq
NM_019487
Target Region
Exon 1~3
Size of Effective Region
~3.4 kb
Overview of Gene Research
Hebp2, also known as heme-binding protein 2, is involved in multiple biological processes. It has been associated with the regulation of microtubule dynamics and mitotic spindle behavior through its interaction with ALG-2, which is significant for genomic stability in cells. Abnormalities in the ALG-2/Hebp2 interaction can lead to improper mitosis [2].
In breast cancer, specifically triple-negative breast cancer (TNBC), overexpression of Hebp2 enhanced the viability of MDA-MB-231 cells in response to cisplatin and BCNU, while CRISPR/Cas9-mediated Hebp2-knockout reduced cell viability in response to these drugs. This indicates that Hebp2 may play a role in resistance to cisplatin and BCNU, which induce DNA cross-links [1]. In ovarian cancer, Hebp2 accelerates cancer progression, modifies the immune landscape, drives macrophage polarization from M0_TAM to M2_TAM, and contributes to carboplatin resistance both in vitro and in vivo [3].
In conclusion, Hebp2 is important in regulating microtubule-related processes during cell division. Its role in cancer, especially in conferring resistance to certain chemotherapeutic drugs in breast and ovarian cancers, makes it a potential biomarker and therapeutic target. The use of gene-editing techniques like CRISPR/Cas9 in cell models has been crucial in uncovering these functions, providing insights into the etiology and potential treatment of related diseases.
References：
1. Kim, Hye Rim, Hong, Jin-Kyung, Kim, Yongsub, Choi, Jeong-Yun. 2024. HEBP2 affects sensitivity to cisplatin and BCNU but not to paclitaxel in MDA-MB-231 breast cancer cells. In Toxicological research, 40, 561-569. doi:10.1007/s43188-024-00249-w. https://pubmed.ncbi.nlm.nih.gov/39345749/
2. Qin, Juan, Yang, Yang, Gao, Siqi, Li, Dengwen, Zhou, Jun. 2017. Deregulated ALG-2/HEBP2 axis alters microtubule dynamics and mitotic spindle behavior to stimulate cancer development. In Journal of cellular physiology, 232, 3067-3076. doi:10.1002/jcp.25754. https://pubmed.ncbi.nlm.nih.gov/28004381/
3. Yang, BiKang, Dai, Miao. 2024. High-dimensional deconstruction of ovarian cancer at single-cell precision reveals HEBP2 that reshape the TIME and drive carboplatin resistance. In Translational oncology, 44, 101917. doi:10.1016/j.tranon.2024.101917. https://pubmed.ncbi.nlm.nih.gov/38554571/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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