C57BL/6JCya-Ostnem1flox/Cya
Common Name
Ostn-flox
Product ID
S-CKO-08178
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-239790-Ostn-B6J-VA
Status
When using this mouse strain in a publication, please cite “Ostn-flox Mouse (Catalog S-CKO-08178) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Ostn-flox
Strain ID
CKOCMP-239790-Ostn-B6J-VA
Gene Name
Product ID
S-CKO-08178
Gene Alias
Ostc
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 16 (Mouse)
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Transcript ID
ENSMUST00000066852
NCBI Transcript ID
NM_198112
Target Region
Exon 3
Size of Effective Region
~1.6 kb
Overview of Gene Research
Ostn, also known as Osteocrin, is a gene encoding a secreted myokine. It plays crucial roles in multiple biological processes. It binds to natriuretic peptide receptor 3 (NPR3), inhibits the degradation of natriuretic peptides, and enhances guanylyl cyclase-A (GC-A/NPR1) signaling [3]. It is involved in processes like bone growth, muscle homeostasis, and energy metabolism, and has implications in various diseases [1,2,3,4,5,6]. Genetic models, such as knockout (KO) mouse models, have been valuable in understanding its functions.
In skeletal muscle, RNA sequencing from wasting skeletal muscles of mice with heart failure showed reduced Ostn expression. Generating skeletal muscle-specific Ostn knockout and overexpressing mice demonstrated that reduced skeletal muscle Ostn levels exaggerate, while its overexpression attenuates cardiac dysfunction and myocardial fibrosis during pressure overload [1]. In adriamycin nephropathy, Ostn-transgenic mice and NPR3 knockout mice showed improvement in phenotypes like albuminuria and podocyte injuries, indicating Ostn works through NPR3. Conversely, Ostn KO mice had increased urinary albumin levels [3]. In the context of cardiac hypertrophy, the expression of Ostn was lower in Ang II-treated mouse heart. Overexpression of Ostn attenuated the hypertrophy and fibrosis of the heart in mice induced by Ang II [4]. For diabetic cardiomyopathy, Ostn expression was reduced in diabetic hearts, and its overexpression significantly attenuated cardiac injury and dysfunction in mice with streptozotocin treatment. OSTN silence aggravated cardiomyocyte apoptosis and injury upon hyperglycemic stimulation in vitro [6].
In conclusion, Ostn is essential in maintaining normal physiological functions in muscles, bones, heart, and kidneys. Studies using Ostn KO or overexpressing mouse models have revealed its significance in disease conditions such as heart failure, nephropathy, cardiac hypertrophy, and diabetic cardiomyopathy. These findings provide potential therapeutic targets for these diseases.
References：
1. Szaroszyk, Malgorzata, Kattih, Badder, Martin-Garrido, Abel, Bauersachs, Johann, Heineke, Joerg. 2022. Skeletal muscle derived Musclin protects the heart during pathological overload. In Nature communications, 13, 149. doi:10.1038/s41467-021-27634-5. https://pubmed.ncbi.nlm.nih.gov/35013221/
2. Wang, Dandan, Tan, Lizhi, Zhi, Yihao, Kang, Xiangtao, Liu, Xiaojun. 2024. Genome-wide variation study and inter-tissue communication analysis unveil regulatory mechanisms of egg-laying performance in chickens. In Nature communications, 15, 7069. doi:10.1038/s41467-024-50809-9. https://pubmed.ncbi.nlm.nih.gov/39152103/
3. Handa, Takaya, Mori, Keita P, Ishii, Akira, Yanagita, Motoko, Yokoi, Hideki. 2021. Osteocrin ameliorates adriamycin nephropathy via p38 mitogen-activated protein kinase inhibition. In Scientific reports, 11, 21835. doi:10.1038/s41598-021-01095-8. https://pubmed.ncbi.nlm.nih.gov/34750411/
4. Ji, Mingyue, Zuo, Zhi, Zhang, Mengyuan, Xu, Zhen, Hu, Guoxin. 2022. Osteocrin alleviates cardiac hypertrophy via attenuating oxidative stress. In Peptides, 152, 170773. doi:10.1016/j.peptides.2022.170773. https://pubmed.ncbi.nlm.nih.gov/35218795/
5. Watanabe-Takano, Haruko, Ochi, Hiroki, Chiba, Ayano, Yasoda, Akihiro, Mochizuki, Naoki. . Mechanical load regulates bone growth via periosteal Osteocrin. In Cell reports, 36, 109380. doi:10.1016/j.celrep.2021.109380. https://pubmed.ncbi.nlm.nih.gov/34260913/
6. Zhang, Xin, Hu, Can, Yuan, Xiao-Pin, Ma, Zhen-Guo, Tang, Qi-Zhu. 2021. Osteocrin, a novel myokine, prevents diabetic cardiomyopathy via restoring proteasomal activity. In Cell death & disease, 12, 624. doi:10.1038/s41419-021-03922-2. https://pubmed.ncbi.nlm.nih.gov/34135313/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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