C57BL/6JCya-Wdr43em1flox/Cya
Common Name
Wdr43-flox
Product ID
S-CKO-15496
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-72515-Wdr43-B6J-VA
Status
When using this mouse strain in a publication, please cite “Wdr43-flox Mouse (Catalog S-CKO-15496) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Wdr43-flox
Strain ID
CKOCMP-72515-Wdr43-B6J-VA
Gene Name
Product ID
S-CKO-15496
Gene Alias
Wrd43, mKIAA0007, 2610318G08Rik
Background
C57BL/6JCya
Gene Full Name
WD repeat domain 43
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 17 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000047086
NCBI Transcript ID
NM_175639
Target Region
Exon 2~3
Size of Effective Region
~2.7 kb
Overview of Gene Research
Wdr43, also known as Utp5 in yeast, is an essential ribogenesis factor. It functions as a chromatin-associated RNA-binding protein (RBP) and release factor, modulating polymerase (Pol) II activity for pluripotency regulation. Wdr43 binds to promoter-associated noncoding/nascent RNAs, occupies gene promoters and enhancers, and interacts with the Pol II machinery [2]. It is also involved in ribosome biogenesis, which is crucial for normal development and cell function [3,5].
In colorectal cancer, Wdr43 knockdown significantly inhibits cell growth by arresting the cell cycle and enhancing the effect of oxaliplatin chemotherapy both in vitro and in vivo. Mechanistically, c-MYC promotes Wdr43 transcription upon oxaliplatin stimulation, and Wdr43 enhances p53 ubiquitination by MDM2, reducing p53 protein stability and promoting cancer cell proliferation and chemoresistance [1].
In non-small cell lung cancer, knocking down Wdr43 expression significantly impairs cell migration, proliferation, and causes cell-cycle arrest in the G1 phase [4].
In colorectal cancer, Wdr43 knockdown increases apoptosis, inhibits cell proliferation, migration, and invasion in vitro and reduces tumorigenesis in animal models, and its effect can be partially reversed by overexpressing vimentin [6].
In conclusion, Wdr43 plays a significant role in ribosome biogenesis and pluripotency regulation. In disease conditions, especially in cancers like colorectal and non-small cell lung cancer, Wdr43 promotes tumor progression. The study of Wdr43 through loss-of-function experiments in cell lines and animal models has provided valuable insights into its role in cancer development, suggesting it could be a potential therapeutic target.
References：
1. Di, Yuqin, Jing, Xiaoqian, Hu, Kunhua, Wang, Ziyang, He, Weiling. 2022. The c-MYC-WDR43 signalling axis promotes chemoresistance and tumour growth in colorectal cancer by inhibiting p53 activity. In Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy, 66, 100909. doi:10.1016/j.drup.2022.100909. https://pubmed.ncbi.nlm.nih.gov/36525936/
2. Bi, Xianju, Xu, Yanhui, Li, Tong, Li, Pilong, Shen, Xiaohua. 2019. RNA Targets Ribogenesis Factor WDR43 to Chromatin for Transcription and Pluripotency Control. In Molecular cell, 75, 102-116.e9. doi:10.1016/j.molcel.2019.05.007. https://pubmed.ncbi.nlm.nih.gov/31128943/
3. Sondalle, S B, Baserga, S J, Yelick, P C. 2016. The Contributions of the Ribosome Biogenesis Protein Utp5/WDR43 to Craniofacial Development. In Journal of dental research, 95, 1214-20. doi:10.1177/0022034516651077. https://pubmed.ncbi.nlm.nih.gov/27221611/
4. Sun, Haijun, Sun, Qi, Qiu, Xie, Li, Aimin, Dai, Jianhua. 2022. WD repeat domain 43 promotes malignant progression of non-small cell lung cancer by regulating CDK2. In The international journal of biochemistry & cell biology, 151, 106293. doi:10.1016/j.biocel.2022.106293. https://pubmed.ncbi.nlm.nih.gov/36041702/
5. Zhao, Chengtian, Andreeva, Viktoria, Gibert, Yann, Baserga, Susan, Yelick, Pamela C. 2014. Tissue specific roles for the ribosome biogenesis factor Wdr43 in zebrafish development. In PLoS genetics, 10, e1004074. doi:10.1371/journal.pgen.1004074. https://pubmed.ncbi.nlm.nih.gov/24497835/
6. Li, Zijian, Feng, Min, Zhang, Jie, Guan, Wenxian, Wang, Hao. 2021. WD40 repeat 43 mediates cell survival, proliferation, migration and invasion via vimentin in colorectal cancer. In Cancer cell international, 21, 418. doi:10.1186/s12935-021-02109-1. https://pubmed.ncbi.nlm.nih.gov/34372874/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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