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Citations
IMMUNITY 47:107 (2017) IF=22.845
You may also be interested in
huPBMC-NKG Mice
Catalog Number: C001329
Genetic Background: NOD-Scid
Strain Description
NKG mice (catalog number: C001316) are a kind of severe immunodeficient mice generated by Cyagen through deleting the Il2rg gene from NOD-Scid mice. NKG mice exhibit deficiency of mature T cells, B cells, and functional NK cells, reduced complement activity, and weak phagocytosis of human-derived cells by macrophages, which are well suited for transplantation of human hematopoietic stem cells (HSC), peripheral blood mononuclear cells (PBMC), adult stem cells and tissues, and patient-derived xenograft (PDX) to obtain the humanized mice with the human immune system.
PBMC humanized mice refer to the immune system humanized mouse model constructed by transplanting human peripheral blood mononuclear cells (PBMC) into immunodeficient mice (such as NKG). NKG mice transplanted with PBMC (huPBMC-NKG) have high efficiency and fast immune reconstitution. The average proportion of human CD45+ in peripheral blood (PB) is about 50% after 3 weeks of transplantation, and the reconstituted immune cells are mainly lymphoid T cells. Human T cells generated after PBMC injection will attack mouse recipient cells, resulting in graft-versus-host disease (GvHD), so this model can be used to evaluate GvHD drugs.
Female NKG mice aged 6 weeks were selected to inject PBMC for the construction of the huPBMC-NKG model, and the construction period was 2-3 weeks.
Short-term studies requiring mature T cells in the fields of tumor immunity, Hematopoiesis, and gene therapy for hematologic and infectious diseases.
1. Survival Curve
Figure 1. The survival curve of huPBMC-NKG mice. Around 35 days post engraftment, huPBMC-NKG mice gradually began to die as Graft-versus-Host Disease (GvHD) appeared. After 60 days of transplantation, about 20% of the mice were still alive.
2. Detection of Human CD45+ Cells in Peripheral Blood (PB)
Figure 2. The proportion of human CD45+ cells in peripheral blood (PB) of huPBMC-NKG mice. After transplantation of human PBMCs, the content of human leukocytes in the peripheral blood gradually increased. After 3 weeks, the average proportion of human CD45+ cells exceeded 40% and the reconstruction speed was relatively fast. After transplantation, the humanization ratio remained at a high level for 3-6 weeks.
3. Detection of Human CD3+ Cells in Peripheral Blood (PB)
Figure 3. The proportion of human CD3+ T cells in the peripheral blood of huPBMC-NKG mice. About 95%- 100% of hCD45+ cells in huPBMC-NKG mice belong to hCD3+ T cells 3 weeks after human PBMC transplantation, indicating that the reconstruction of the human immune system in huPBMC-NKG mice was dominated by T cells.
4. The Graft-versus-Host Disease (GvHD)
Figure 4. GvHD scores and body weight changes in NKG mice after PBMC transplantation. GvHD symptoms begin to appear around 28d. Mice lost a significant amount of body weight and the body weight was reduced by more than 20% after 45d.
