Rag1 KO Mouse
Product Name
Rag1 KO Mouse
Product ID
C001197
Strain Name
C57BL/6NCya-Rag1em1/Cya
Backgroud
C57BL/6NCya
Status
When using this mouse strain in a publication, please cite “Rag1 KO Mouse (Catalog C001197) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Gene Name
Rag1
Gene Alias
Rag-1
NCBI ID
Chromosome
Chr 2 (Mouse)
MGI ID
Datasheet
Strain Description
The V(D)J recombination activation gene RAG1 was isolated based on its ability to activate V(D)J recombination on an artificial substrate in fibroblasts. This property and the expression pattern in tissues and cell lines indicate that RAG1 either activates or catalyzes the V(D)J recombination reaction of immunoglobulin and T cell receptor genes [1]. Diseases associated with RAG1 include Alpha/Beta T-cell Lymphopenia With Gamma/Delta T-cell Expansion, Severe Cytomegalovirus Infection, Autoimmunity, and Combined Cellular And Humoral Immune Defects With Granulomas [2].
The Rag1-KO Mouse is a RAG1-deficient model that exhibits smaller lymphoid organs and does not contain mature B and T lymphocytes, the arrest of B and T cell differentiation occurs at an early stage and correlates with the inability to perform V(D)J recombination. The homozygote Rag1-KO mice developed normally and were fertile.
Reference
Mombaerts P, Iacomini J, Johnson RS, Herrup K, Tonegawa S, Papaioannou VE. RAG-1-deficient mice have no mature B and T lymphocytes. Cell. 1992 Mar 6;68(5):869-77.
Bosticardo M, Pala F, Notarangelo LD. RAG deficiencies: Recent advances in disease pathogenesis and novel therapeutic approaches. Eur J Immunol. 2021 May;51(5):1028-1038.
Strain Strategy
The Rag1 gene is located on chromosome 2 of the mouse and contains 2 exons, the start codon ATG and the stop codon TAA are both located in Exon 2, and Exon 2 of the Rag1 gene is knocked out by gene editing technology.
Figure 1. Diagram of the gene editing strategy for the generation of Rag1 KO mice.
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