This strain is a human ACE2 (hACE2) conditional overexpression model generated by integrating the hACE2 gene expression element into the mouse ROSA26 safe harbor site. Under normal conditions, the expression of hACE2 is blocked by the upstream loxP-Stop-loxP expression stop cassette. Upon mating with Cre mice, the Cre recombinase mediates the deletion of the sequence between the loxP sites, including the stop cassette, thereby enabling the expression of hACE2. When bred with tissue-specific Cre mice, overexpression of the hACE2 gene and protein can be achieved in specific tissues of the offspring mice. Homozygous ROSA26-LSL-hACE2 mice are viable and fertile.