H11-CAG-LSL-hMYC-IRES-EGFP Mouse
Product Name
H11-CAG-LSL-hMYC-IRES-EGFP Mouse
Product ID
C001338
Strain Name
C57BL/6JCya-lgs2em1(CAG-LSL-HA tag-hMYC-IRES-eGFP)/Cya
Backgroud
C57BL/6JCya
When using this mouse strain in a publication, please cite “H11-CAG-LSL-hMYC-IRES-EGFP Mouse (Catalog C001338) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Gene Name
Gene Alias
MRTL, MYCC, c-Myc, bHLHe39
NCBI ID
Chromosome
Chr 8
MGI ID
Datasheet
Strain Description
The MYC gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis, and cellular transformation. The MYC protein forms a heterodimer with the related transcription factor MAX, this complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of the MYC gene is frequently observed in numerous human cancers, MYC protein regulates the expression of a variety of genes related to cell proliferation and metabolic processes, and the MYC gene is also the most common high-abundance oncogene in human cancers. Translocations involving the MYC gene are associated with Burkitt lymphoma and multiple myeloma in human patients [1-3]. The H11 safe harbor locus is located on mouse chromosome 11 and is capable of integrating exogenous genes driven by a designated promoter for stable expression. This strain carries a CAG promoter-driven LSL-hMYC-IRES-EGFP conditional overexpression structure inserted at the H11 safe harbor locus and overexpresses the human MYC gene in specific tissues or cells after mating with tissue-specific Cre mice.
Reference
Mice Expressing Myc in Neural Precursors Develop Choroid Plexus and Ciliary Body Tumors[J]. American Journal of Pathology Official Publication of the American Association of Pathologists, 2018.
Altho Ff K ,Be Ckers A ,Be Ll E , et al. A Cre-conditional MYCN-driven neuroblastoma mouse model as an improved tool for preclinical studies[J]. Oncogene, 2014, 34(26):3357.
Bj Rn K ,Tiziana M ,Sarah N , et al. The ERBB network facilitates KRAS-driven lung tumorigenesis[J]. Science Translational Medicine, 2018, 10(446):S96-S96.
Strain Strategy
Insertion of the “CAG-LSL-hMYC-IRES-EGFP” gene expression element into the H11 safe harbor locus in the mouse genome.
Figure 1. Diagram of the gene editing strategy for the generation of H11-CAG-LSL-hMYC-IRES-EGFP mice.
Application Area
The model can be mated with different tissue-specific Cre mice for the study of tumors in different tissues or organs.
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