H11-CAG-MerCreMer Mouse
Product Name
H11-CAG-MerCreMer Mouse
Product ID
C001356
Strain Name
C57BL/6JCya-Igs2em1(CAG-MerCreMer)/Cya
Backgroud
C57BL/6JCya
Examples of Expressing Tissues/Cells
Systemic
When using this mouse strain in a publication, please cite “H11-CAG-MerCreMer Mouse (Catalog C001356) were purchased from Cyagen.”
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Strain Description
The CAG promoter is an artificially constructed combination promoter consisting of the cytomegalovirus (CMV) early enhancer element and the chicken beta-actin promoter, commonly used to drive high-level expression of genes in expression vectors in mammals.
The H11-CAG-MerCreMer mouse is a tamoxifen-inducible tool mouse that ubiquitously expresses the MerCreMer recombinase under the control of the CAG promoter. Compared to Cre recombinase fused with a single estrogen receptor (CreMer or CreERT), the double ER-fusion Cre recombinase (MerCreMer) exhibits higher Cre recombinase activity and less expression leakage. In the absence of tamoxifen, MerCreMer recombinase remains in the cytoplasm. Upon tamoxifen treatment, the recombinase translocates into the nucleus to exert its recombination function. When crossed with mice harboring loxP sites, the offspring will undergo Cre-mediated recombination between loxP sites in a wide range of cells and tissues after tamoxifen induction. This strain is homozygous viable.
Strain Strategy
The CAG-MerCreMer gene expression cassette was integrated into the H11 safe harbor locus of the mouse genome through gene-editing techniques.
Figure 1. Diagram of the gene editing strategy for the generation of H11-CAG-MerCreMer mice.
Application Area
This tamoxifen-inducible mouse can serve as a Cre recombinase tool for systemic loxP recombination. For example, crossing this mouse with conditional knockout mice allows for systemic deletion of sequences between two loxP sites.
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