The Cyp19a1-IRES-Cre mice were constructed using gene editing technology to insert an IRES-Cre element downstream of the stop codon of the endogenous Cyp19a1 gene in mice. The expression pattern of Cre recombinase is similar to that of the endogenous gene and does not affect its normal expression. When this strain is crossed with mice containing loxP sites, sequence recombination between loxP sites mediated by Cre recombinase can occur in the granulosa cells of the ovaries of offspring mice.