Elf5-Cre Mouse
Product Name
Elf5-Cre Mouse
Product ID
C001389
Strain Name
C57BL/6JCya-Elf5em1(P2A-Cre)/Cya
Backgroud
C57BL/6JCya
When using this mouse strain in a publication, please cite “Elf5-Cre Mouse (Catalog C001389) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Gene Name
Gene Alias
ESE-2, ESE-5, ESE-5.
NCBI ID
Chromosome
Chr 2
MGI ID
Datasheet
Strain Description
The E26 transforming sequence (ETS) transcription factor family plays a key role in the embryogenesis or determination of cell subtypes in the endocellular clusters during mammary gland development. The E74-like ETS transcription factor 5 (ELF5) gene encodes a member of the epithelial-specific ETS family of transcription factors. In addition to playing a role in the late stages of terminal differentiation of keratinocytes, ELF5 also regulates many epithelial-specific genes in tissues containing glandular epithelium, such as the salivary glands and prostate [1]. ELF5 is an important regulator in the transformation and differentiation of mammary cell subtypes and is also considered a tumor suppressor transcription factor in breast cancer [2-3].
The Elf5-Cre mice were constructed using gene editing technology to insert the P2A-Cre expression element into the endogenous Elf5 gene of mice. The expression pattern of Cre recombinase is similar to that of the endogenous gene. When this strain is crossed with mice containing loxP sites, sequence recombination between loxP sites mediated by Cre recombinase can occur in tissues such as the mammary gland and uterus of offspring mice. The heterozygotes are viable and fertile.
Reference
Lee HJ, Ormandy CJ. Elf5, hormones and cell fate. Trends Endocrinol Metab. 2012 Jun;23(6):292-8.
Qu X, Li Q, Tu S, Yang X, Wen W. ELF5 inhibits the proliferation and invasion of breast cancer cells by regulating CD24. Mol Biol Rep. 2021 Jun;48(6):5023-5032.
Piggin CL, Roden DL, Gallego-Ortega D, Lee HJ, Oakes SR, Ormandy CJ. ELF5 isoform expression is tissue-specific and significantly altered in cancer. Breast Cancer Res. 2016 Jan 7;18(1):4.
Strain Strategy
The TGA stop codon was replaced with the P2A-Cre-WPRE-rBG pA cassette.
Figure 1. Diagram of the gene editing strategy for the generation of Elf5-Cre mice.
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