B6-hCALCRL Mouse
Product Name
B6-hCALCRL Mouse
Product ID
C001497
Strain Name
C57BL/6JCya-Calcrlem1(hCALCRL)/Cya
Backgroud
C57BL/6JCya
When using this mouse strain in a publication, please cite “B6-hCALCRL Mouse (Catalog C001497) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Gene Name
Gene Alias
CRLR, CGRPR, LMPHM8
NCBI ID
Chromosome
Chr 2
MGI ID
Datasheet
Strain Description
Calcitonin receptor-like receptor (CALCRL) is a seven-transmembrane G protein-coupled receptor encoded by the CALCRL gene. It mediates the pleiotropic effects of calcitonin gene-related peptide (CGRP) and adrenal medullary peptide (ADM), two structurally related neuropeptides that are potent vasodilators and play an important role in blood pressure regulation [1]. In addition, CALCRL is involved in various other biological processes, including cell proliferation, cell death regulation, vascular biology, and inflammation [2]. CALCRL is currently being investigated as a new target for the treatment of migraine [3]. In solid tumors, antibodies that target CALCRL have been shown to reduce tumor growth by either disrupting angiogenesis or by directly inhibiting cancer cell proliferation [4]. CALCRL is also expressed in normal CD34+ hematopoietic progenitor cells, and CGRP and ADM can directly stimulate these cells to form colonies in vitro, suggesting a role for CALCRL in physiological bone marrow generation [5].
This strain represents a humanized mouse model of the Calcrl gene. Using gene editing technology, the sequence encoding the extracellular domain of the mouse Calcrl gene was replaced with the corresponding sequence from the human CALCRL gene. This model can be used to study the mechanisms of various physiological and pathological processes, such as blood pressure regulation, cell proliferation, cell death, vascular biology, physiological bone marrow generation, inflammation, and tumor growth, as well as the development of CALCRL-targeted migraine drugs and therapies. Homozygous B6-hCALCRL mice are viable and fertile.
Reference
Brain SD, Williams TJ, Tippins JR, Morris HR, MacIntyre I. Calcitonin gene-related peptide is a potent vasodilator. Nature. 1985 Jan 3-9;313(5997):54-6.
Russell FA, King R, Smillie SJ, Kodji X, Brain SD. Calcitonin gene-related peptide: physiology and pathophysiology. Physiol Rev. 2014 Oct;94(4):1099- 142.
Hershey AD. CGRP - The Next Frontier for Migraine. N Engl J Med. 2017 Nov 30;377(22):2190-2191.
Toda M, Suzuki T, Hosono K, Hayashi I, Hashiba S, Onuma Y, Amano H, Kurihara Y, Kurihara H, Okamoto H, Hoka S, Majima M. Neuronal system-dependent facilitation of tumor angiogenesis and tumor growth by calcitonin gene-related peptide. Proc Natl Acad Sci U S A. 2008 Sep 9;105(36):13550-5.
Chute JP, Muramoto GG, Dressman HK, Wolfe G, Chao NJ, Lin S. Molecular profile and partial functional analysis of novel endothelial cell-derived growth factors that regulate hematopoiesis. Stem Cells. 2006 May;24(5):1315-27.
Strain Strategy
The sequence encoding the extracellular domain of the endogenous mouse Calcrl gene was replaced with the corresponding sequence from the human CALCRL gene, while preserving the sequence encoding the signal peptide of the mouse Calcrl gene.
Figure 1. Gene editing strategy for B6-hCALCRL mice.
Application Area
Drug discovery and screening for migraine treatment;
Efficacy and safety evaluation of migraine treatment drugs;
Vascular biology and blood pressure regulation research;
Cell proliferation and apoptosis research;
Tumor growth inhibition and inflammation research;
Hematopoietic stem/progenitor cell generation and differentiation research.
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