The B6-hCXCR2 mouse is a humanized model constructed using gene editing technology to replace the entire base sequence of the mouse Cxcr2 gene in situ with the corresponding sequence from the human CXCR2 gene. Homozygous B6-hCXCR2 mice are viable and fertile. This model can be used for studying the pathological mechanisms and therapeutic approaches of inflammatory diseases and cancers, and for the development of CXCR2-targeted drugs.