RCL-tdTomato Mouse
Product Name
RCL-tdTomato Mouse
Product ID
C001742
Strain Name
C57BL/6JCya-Gt(ROSA)26Sorem1(CAG-LSL-tdTomato)/Cya
Backgroud
C57BL/6JCya
Status
When using this mouse strain in a publication, please cite “RCL-tdTomato Mouse (Catalog C001742) were purchased from Cyagen.”
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Strain Description
This mouse strain is a fluorescent reporter mouse carrying a red fluorescent protein (tdTomato) gene expression cassette, with tdTomato protein expression regulated by the CAG promoter. The CAG promoter is a modified composite promoter consisting of the chicken β-actin gene promoter, the cytomegalovirus (CMV) early enhancer, the rabbit β-actin gene splice acceptor, and the bovine globulin polyA signal sequence. Renowned for driving robust and stable gene expression in multiple mammalian cell types, this promoter induces consistent tdTomato expression across systemic tissues and organs.
RCL-tdTomato mice are generated via genome editing to knock the CAG-LSL-tdTomato cassette into the ROSA26 safe harbor locus. Upstream of the tdTomato gene lies a loxP-Stop-loxP (LSL) cassette, which blocks the transcription and expression of tdTomato. Upon Cre recombination, cells expressing Cre recombinase (and their future cell lineages) exhibit strong tdTomato fluorescence. This model is suitable for validating Cre mice with high recombination efficiency and potential expression leakage, as well as for other cell lineage tracing applications.
Strain Strategy
The CAG promoter-loxP-PGK-Neo-6*SV40 pA-loxP-Kozak-tdTomato-WPRE-BGH pA cassette was cloned into intron 1 of ROSA26.
Figure 1. Gene editing strategy of RCL-tdTomato Mice.
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