Ccr7-EGFP-loxP-Stop-loxP-DTR
Product ID:
C001749
Background:
C57BL/6JCya
Documents:
Expressing Tissues/Cells:
T cells, dendritic cells, B cells, NK cells, spleen, and Ccr7-positive cells
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Strain Description
The TAG stop codon was replaced with “P2A-EGFP-IRES-loxP-6xSV40 pA-loxP-DTR” cassette. Upstream of the DTR (diphtheria toxin receptor) gene lies a loxP-Stop-loxP cassette that blocks DTR transcription and expression. In the absence of Cre recombinase, the Ccr7 promoter drives EGFP expression, labeling Ccr7-positive cells with green fluorescence. Upon Cre recombination, Cre-mediated deletion of the loxP-flanked stop (LSL) element enables DTR expression in Cre-positive cells. Intravenous administration of diphtheria toxin (DT) then selectively ablates these cells.
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