Leng8-3xFLAG tag-EGFP-iCre Mouse
Product Name
Leng8-3xFLAG tag-EGFP-iCre Mouse
Product ID
C001758
Strain Name
C57BL/6JCya-Leng8em1(3xFLAG tag-EGFP-iCre)/Cya
Backgroud
C57BL/6JCya
Examples of Expressing Tissues/Cells
Neocortex, hippocampus, spleen, adipose tissue, kidney, and Leng8-positive cells
When using this mouse strain in a publication, please cite “Leng8-3xFLAG tag-EGFP-iCre Mouse (Catalog C001758) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Gene Name
Gene Alias
--
NCBI ID
Chromosome
Chr 7
MGI ID
Datasheet
Strain Description
The TAG stop codon was replaced with the 3xFLAG tag-3xEAAAK-EGFP-IRES-iCre cassette. iCre recombinase and enhanced green fluorescent protein (EGFP) are expressed under the regulatory control of Leng8 gene elements. The 3xFLAG tag is a tag consisting of three tandem FLAG epitopes (DYKDDDDK). When inserted into a target gene, it allows for efficient detection, localization, or purification of the target protein using anti-FLAG antibodies. In addition to studies of Cre recombinase-mediated gene recombination, this model can also be used for EGFP fluorescent protein tracing studies.
Strain Strategy
Figure 1. Gene editing strategy for Leng8-3xFLAG tag-EGFP-iCre mice.
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