Piwil1-EGFP-iCre
Product ID:
C001762
Background:
C57BL/6JCya
Documents:
Expressing Tissues/Cells:
Spermatocytes, testis, hippocampus, prefrontal cortex, cerebellum, and Piwil1-positive cells
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Model Name
Piwil1-EGFP-iCre
Product ID
C001762
Gene Name
Background
C57BL/6JCya
Gene Alias
MIWI
Classification
Cre Driver Lines,Reporter Mouse Lines
NCBI ID
Phenotype
Chromosome
5
Document
Note: When using this mouse strain in a publication, please cite “Piwil1-EGFP-iCre mice (Catalog C001762) were purchased from Cyagen.”
Strain Description
Piwil1-EGFP-iCre mice are generated via genome editing by replacing the TAA stop codon of the mouse Piwil1 gene with an HA tag-3xEAAAK-EGFP-IRES-iCre cassette, enabling expression of iCre recombinase (codon-optimized Cre) and EGFP under the control of endogenous Piwil1 regulatory elements. The expression patterns of iCre recombinase mirror that of the endogenous Piwil1 gene. When crossed with mice containing loxP sites, Cre-mediated recombination between loxP sites is expected to occur in Piwil1-positive cells of the offspring. Beyond Cre-loxP gene recombination, this model allows EGFP-based fluorescent tracing.
Validation Data