The B6-hTSLP mouse is a humanized model constructed using gene editing technology, where the mouse Tslp endogenous domain (aa.20~140) was replaced with the human TSLP domain (aa.29~159). The murine signal peptide (aa.1~19) was preserved. This model can be used for studying the pathological mechanisms and therapeutic approaches of allergic and inflammatory diseases and for the development of TSLP-targeted drugs.