Il4 KO Mouse
Product Name
Il4 KO Mouse
Product ID
C001818
Strain Name
C57BL/6JCya-Il4em1/Cya
Backgroud
C57BL/6JCya
Status
When using this mouse strain in a publication, please cite “Il4 KO Mouse (Catalog C001818) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Gene Name
Il4
Gene Alias
Il-4, BSF-1
NCBI ID
Chromosome
Chr 11 (Mouse)
MGI ID
Datasheet
Strain Description
The IL4 gene encodes interleukin-4 (IL-4), a multifunctional cytokine primarily secreted by Th2 cells, mast cells, and eosinophils. IL-4 promotes Th2 cell differentiation, B cell class switching to IgE, and alternative activation of macrophages (M2 polarization), playing critical roles in allergic responses, anti-parasitic immunity, and tissue repair. IL-4 and its receptor IL-4R are key regulators of immune responses and inflammation; dysregulation of this pathway is associated with various pathologies, including allergic diseases (e.g., asthma, atopic dermatitis, allergic rhinitis), parasitic infections, and certain cancers [1-5].
The Il4 KO mouse, a gene knockout model generated by deleting exons 1–4 of the murine Il4 gene (homologous to human IL4), serves as a valuable tool for investigating the pathogenesis of allergic disorders (e.g., asthma, atopic dermatitis, allergic rhinitis), anti-parasitic immunity, and specific cancers, as well as for developing related therapeutic strategies.
Reference
Gandhi NA, Bennett BL, Graham NM, Pirozzi G, Stahl N, Yancopoulos GD. Targeting key proximal drivers of type 2 inflammation in disease. Nat Rev Drug Discov. 2016 Jan;15(1):35-50.
Oetjen LK, Mack MR, Feng J, Whelan TM, Niu H, Guo CJ, Chen S, Trier AM, Xu AZ, Tripathi SV, Luo J, Gao X, Yang L, Hamilton SL, Wang PL, Brestoff JR, Council ML, Brasington R, Schaffer A, Brombacher F, Hsieh CS, Gereau RW 4th, Miller MJ, Chen ZF, Hu H, Davidson S, Liu Q, Kim BS. Sensory Neurons Co-opt Classical Immune Signaling Pathways to Mediate Chronic Itch. Cell. 2017 Sep 21;171(1):217-228.e13.
Bankaitis KV, Fingleton B. Targeting IL4/IL4R for the treatment of epithelial cancer metastasis. Clin Exp Metastasis. 2015 Dec;32(8):847-56.
Davoodi P, Mahesh PA, Holla AD, Ramachandra NB. A preliminary study on the association of single nucleotide polymorphisms of interleukin 4 (IL4), IL13, IL4 receptor alpha (IL4Rα) & Toll-like receptor 4 (TLR4) genes with asthma in Indian adults. Indian J Med Res. 2015 Dec;142(6):675-80.
Choy DF, Hart KM, Borthwick LA, Shikotra A, Nagarkar DR, Siddiqui S, Jia G, Ohri CM, Doran E, Vannella KM, Butler CA, Hargadon B, Sciurba JC, Gieseck RL, Thompson RW, White S, Abbas AR, Jackman J, Wu LC, Egen JG, Heaney LG, Ramalingam TR, Arron JR, Wynn TA, Bradding P. TH2 and TH17 inflammatory pathways are reciprocally regulated in asthma. Sci Transl Med. 2015 Aug 19;7(301):301ra129.
Strain Strategy
The mouse Il4 gene consists of 4 exons, with the ATG start codon in exon 1 and the TAG stop codon in exon 4. This strain was generated by deleting the region spanning exons 1–4 via genome editing.
Figure 1. Diagram of the gene editing strategy for the generation of Il4 KO mice.
Application Area
Pathogenesis and therapeutic evaluation of allergic diseases (e.g., asthma, atopic dermatitis, allergic rhinitis);
Construction of anti-parasitic infection models and dissection of defense mechanisms;
Regulatory mechanisms of Th1/Th2 immune balance;
Modulation of tumor immune microenvironment and development of immunotherapeutic strategies.
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