huMST1 Mouse
Product Name
huMST1 Mouse
Product ID
C002070
Strain Name
C57BL/6NCya-Mst1tm1(hMST1)/Cya
Background
C57BL/6NCya
Status
When using this mouse strain in a publication, please cite “huMST1 Mouse (Catalog C002070) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Standard products are sold as live mice derived from cryorecovery or expansion. Frozen materials are available upon request. Pricing and lead times depend on real-time inventory.
Gene Name
MST1
Gene Alias
MSP, HGFL, NF15S2, D3F15S2, DNF15S2
NCBI ID
Chromosome
Chr 3 (Human)
MGI ID
Datasheet
Strain Description
MST1 (Macrophage stimulating 1), also known as hepatocyte growth factor-like protein (HGFL) or macrophage-stimulating protein (MSP), is a secreted growth factor belonging to the kringle protein family and is homologous to hepatocyte growth factor (HGF) [1–2]. It is predominantly expressed in the liver and regulates macrophage chemotaxis, phagocytosis, morphological changes, and the production of inflammatory mediators by binding to its receptor RON (MST1R). MST1 also plays a role in physiological processes such as ciliary motility [3–4]. The nonsynonymous coding variant rs3197999 (R689C) in the MST1 gene is significantly associated with inflammatory bowel disease (IBD), including Crohn’s disease and ulcerative colitis, as well as primary sclerosing cholangitis (PSC) [5–6].
The huMST1 mouse model is a humanized model generated using gene-editing technology, in which the mouse Mst1 endogenous genomic DNA was replaced with the human MST1 genomic DNA while retaining the murine signal peptide sequence. This model is primarily applicable to mechanistic studies of inflammatory bowel disease (IBD), primary sclerosing cholangitis, macrophage-associated inflammation, and immune regulation, as well as the exploration of therapeutic strategies targeting the MST1/MSP–RON pathway. It can be used to evaluate the potential effects of related drugs in inflammatory and immune diseases.
Reference
Yoshimura, T., Yuhki, N., Wang, M H., Skeel, A., & Leonard, E J.. (1993). Cloning, sequencing, and expression of human macrophage stimulating protein (MSP, MST1) confirms MSP as a member of the family of kringle proteins and locates the MSP gene on chromosome 3. The Journal of biological chemistry, 268(21), 15461-8.
Han, S., Stuart, L A., & Degen, S J.. (1991). Characterization of the DNF15S2 locus on human chromosome 3: identification of a gene coding for four kringle domains with homology to hepatocyte growth factor. Biochemistry, 30(40), 9768-80.
Skeel, A., Yoshimura, T., Showalter, S D., Tanaka, S., & Appella, E.. (1991). Macrophage stimulating protein: purification, partial amino acid sequence, and cellular activity. The Journal of experimental medicine, 173(5), 1227-34.
Sakamoto, O., Iwama, A., Amitani, R., Takehara, T., & Yamaguchi, N.. (1997). Role of macrophage-stimulating protein and its receptor, RON tyrosine kinase, in ciliary motility. The Journal of clinical investigation, 99(4), 701-9.
Goyette, P., Lefebvre, C., Ng, A., Brant, S R., & Cho, J H.. (2008). Gene-centric association mapping of chromosome 3p implicates MST1 in IBD pathogenesis. Mucosal immunology, 1(2), 131-8.
Melum, Espen., Franke, Andre., Schramm, Christoph., Weismüller, Tobias J., & Gotthardt, Daniel Nils.. (2010). Genome-wide association analysis in primary sclerosing cholangitis identifies two non-HLA susceptibility loci. Nature genetics, 43(1).
Strain Strategy
The mouse Mst1 endogenous genomic region was replaced with the human MST1 genomic region. The murine signal peptide was preserved. The KI region may affect the expression of mouse Mir7088, while its effect on mouse Rnf123 remains unclear.
Figure 1. Gene editing strategy of huMST1 mice.
Application Area
Mechanistic studies of inflammatory bowel disease (IBD), including Crohn’s disease and ulcerative colitis, primary sclerosing cholangitis (PSC), macrophage-associated inflammation, and immune regulation;
Exploration of therapeutic strategies targeting the MST1/MSP–RON pathway.
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