hCD5 Mouse
Product Name
hCD5 Mouse
Product ID
C002093
Strain Name
C57BL/6NCya-Cd5tm1(hCD5)/Cya
Backgroud
C57BL/6NCya
Status
When using this mouse strain in a publication, please cite “hCD5 Mouse (Catalog C002093) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Gene Name
CD5
Gene Alias
T1, LEU1
NCBI ID
Chromosome
Chr 11 (Human)
MGI ID
Datasheet
Strain Description
CD5 (Cluster of Differentiation 5) is a type I transmembrane glycoprotein belonging to the scavenger receptor cysteine-rich (SRCR) superfamily. It is primarily expressed on the surface of T cells and a subset of B cells. In T-cell malignancies, CD5 has emerged as an important target for emerging immunotherapeutic approaches, including CAR-T cells, CAR-γδT cells, and NK cell engagers (NKCEs) [1-2]. In addition, the involvement of CD5 in immune tolerance, inflammatory regulation, and tumor immune microenvironment modulation makes it an important target for basic immunology and immune regulatory mechanism studies [3].
hCD5 mice were generated using gene editing technology, in which the partial sequence of exon 2 and partial sequence of intron 2 of the mouse Cd5 gene were replaced with the Chimeric human CD5 CDS-3'UTR of mouse Cd5-WPRE-BGH pA cassette. The signal peptide-encoding sequence of the mouse Cd5 gene was retained. hCD5 mice are mainly used for cancer immunotherapy and autoimmune disease research. This model can be applied to evaluate the in vivo efficacy of anti-human CD5 antibodies and other CD5-targeted therapeutic strategies, support mechanism studies of T-cell malignancies, and facilitate the development and evaluation of emerging immunotherapeutic approaches, including CAR-T cells, CAR-γδT cells, and NK cell engagers (NKCEs). Furthermore, this model can be used to investigate the roles of CD5 in T-cell activation, immune tolerance, inflammatory responses, and tumor immune microenvironment regulation, as well as to support studies of immune-modulatory therapeutic strategies for autoimmune diseases.
Reference
Zhu Z, Li H, Lu Q, Zhang Z, Li J, Wang Z, Yang N, Yu Z, Yang C, Chen Y, Lu H, Wang W, Niu T, Nie C, Tong A. mRNA-Engineered CD5-CAR-γδTCD5- Cells for the Immunotherapy of T-Cell Acute Lymphoblastic Leukemia. Adv Sci (Weinh). 2024 Sep;11(35):e2400024.
Yang C, Wang P, Yang M, Lu H, Lu Q, Zhang Z, Wang Z, Zhu Z, Li H, Zeng W, Yu Z, Li M, Zhao L, Ling B, Wang W, Yi H, Xiong Y, Niu T, Tong A. A nanobody-based tri-specific NK cell engager targeting CD5 triggers antitumor immunity. Cell Rep Med. 2025 Oct 21;6(10):102409.
Dalloul A. CD5: a safeguard against autoimmunity and a shield for cancer cells. Autoimmun Rev. 2009 Feb;8(4):349-53.
Strain Strategy
The partial sequence of exon 2 and partial sequence of intron 2 of the mouse Cd5 gene were replaced with the Chimeric human CD5 CDS-3'UTR of mouse Cd5-WPRE-BGH pA cassette. The signal peptide-encoding sequence of the mouse Cd5 gene was retained.
Figure 1. Gene editing strategy of hCD5 mice.
*Chimeric human CD5 CDS: extracellular domain of human CD5 + transmembrane domian and cytoplasmic domian of mouse Cd5
Application Area
Cancer immunotherapy research: Used to evaluate the in vivo efficacy of anti-human CD5 antibodies and other CD5-targeted therapeutic strategies, supporting the development and evaluation of CD5-targeted therapeutics;
T-cell malignancy research: Used for investigating disease mechanisms of T-cell malignancies, including T-cell acute lymphoblastic leukemia (T-ALL) and peripheral T-cell lymphoma (PTCL), as well as evaluating CD5-targeted therapeutic strategies;
Emerging cellular immunotherapy research: Used for the development and in vivo evaluation of novel immunotherapeutic approaches, including CAR-T cells, CAR-γδT cells, and NK cell engagers (NKCEs);
Autoimmune and basic immunology research: Used to investigate the roles of CD5 in T-cell activation, immune tolerance, inflammatory responses, and tumor immune microenvironment regulation, and to support mechanism studies and evaluation of immunomodulatory therapeutic strategies for autoimmune diseases, including rheumatoid arthritis (RA) and multiple sclerosis (MS).
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