huGRB14 Mouse
Product Name
huGRB14 Mouse
Product ID
C002096
Strain Name
C57BL/6NCya-Grb14tm1(hGRB14)/Cya
Backgroud
C57BL/6NCya
Status
When using this mouse strain in a publication, please cite “huGRB14 Mouse (Catalog C002096) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Gene Name
GRB14
Gene Alias
--
NCBI ID
Chromosome
Chr 2 (Human)
MGI ID
Datasheet
Strain Description
GRB14 (Growth factor receptor-bound protein 14) is a member of the GRB7 protein family and functions as an intracellular adaptor protein that regulates multiple signaling pathways through interactions with receptor tyrosine kinases and downstream signaling molecules. GRB14 is an important negative regulator of insulin receptor signaling by binding to the insulin receptor and inhibiting its kinase activity, thereby contributing to the regulation of glucose and lipid metabolic homeostasis. Studies have shown that abnormal GRB14 expression is associated with insulin resistance, type 2 diabetes (T2D), hypertriglyceridemia, metabolic dysfunction-associated steatotic liver disease (MASLD), and other metabolic disorders [1-4]. In addition, dysregulated GRB14 expression has been implicated in the development of various cancers and may regulate malignant biological processes, including tumor cell proliferation, migration, and invasion [5-6].
The huGRB14 humanized mouse model was generated using gene-editing technology by replacing the mouse Grb14 gene sequence from the start codon to the stop codon with the corresponding human GRB14 gene sequence from the start codon to the stop codon. This model can be used for mechanistic studies of metabolic disorders, including hypertriglyceridemia, type 2 diabetes (T2D), and metabolic dysfunction-associated steatotic liver disease (MASLD), as well as investigations into GRB14-associated tumor development. Furthermore, this model may facilitate the evaluation of potential glucose-lowering, lipid-modulating, and anti-tumor therapeutic strategies targeting human GRB14. It can also serve as an immunization tool mouse model for the generation of human GRB14-specific monoclonal antibodies.
Reference
Gondoin A, Morzyglod L, Desbuquois B, Burnol AF. Contrôle de la signalisation et de l'action de l'insuline par la protéine Grb14 [Control of insulin signalisation and action by the Grb14 protein]. Biol Aujourdhui. 2014;208(2):119-36. French.
Cariou B, Bereziat V, Moncoq K, Kasus-Jacobi A, Perdereau D, Le Marcis V, Burnol AF. Regulation and functional roles of Grb14. Front Biosci. 2004 May 1;9:1626-36.
Holt LJ, Siddle K. Grb10 and Grb14: enigmatic regulators of insulin action--and more? Biochem J. 2005 Jun 1;388(Pt 2):393-406.
Chen Y, Du X, Kuppa A, et al. Genome-wide association meta-analysis identifies 17 loci associated with nonalcoholic fatty liver disease. Nat Genet. 2023;55(10):1640-1650.
Gu CB, Wang C. GRB14: A prognostic biomarker driving tumor progression in gastric cancer through the PI3K/AKT signaling pathway by interacting with COBLL1. Open Life Sci. 2025 Apr 29;20(1):20251084.
Hu H, Feng X, Zou Y, Jia X, Yang H, Luo X. High expression of GRB14 is associated with cancer progression and poor prognosis in gastric cancer. Discov Oncol. 2025 Nov 18;16(1):2117.
Strain Strategy
The mouse Grb14 gene sequence from the start codon to the stop codon was replaced with the corresponding human GRB14 gene sequence.
Figure 1. Gene editing strategy of huGRB14 mice.
Application Area
Metabolic disease research: Used for the research on the mechanisms of metabolic diseases such as hypertriglyceridemia, type 2 diabetes (T2D), and metabolic dysfunction-associated steatotic liver disease (MASLD), and for drug evaluation;
Cancer research: Used for the research on the mechanisms of GRB14-related tumorigenesis and development, and for the evaluation of anti-cancer treatment strategies;
Antibody development: Used for the preparation of anti-human GRB14 specific monoclonal antibodies.
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
