huMST1(2) Mouse
Product Name
huMST1(2) Mouse
Product ID
C002110
Strain Name
C57BL/6Cya-Mst1em1(hMST1)/Cya
Background
C57BL/6Cya
Status
When using this mouse strain in a publication, please cite “huMST1(2) Mouse (Catalog C002110) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Standard products are sold as live mice derived from cryorecovery or expansion. Frozen materials are available upon request. Pricing and lead times depend on real-time inventory.
Gene Name
MST1
Gene Alias
MSP, HGFL, NF15S2, D3F15S2, DNF15S2
NCBI ID
Chromosome
Chr 3 (Human)
MGI ID
Datasheet
Strain Description
MST1 (Macrophage stimulating 1), also known as hepatocyte growth factor-like protein (HGFL) or macrophage-stimulating protein (MSP), is a secreted growth factor belonging to the kringle protein family and is homologous to hepatocyte growth factor (HGF) [1–2]. It is predominantly expressed in the liver and regulates macrophage chemotaxis, phagocytosis, morphological changes, and the production of inflammatory mediators by binding to its receptor RON (MST1R). MST1 also plays a role in physiological processes such as ciliary motility [3–4]. The nonsynonymous coding variant rs3197999 (R689C) in the MST1 gene is significantly associated with inflammatory bowel disease (IBD), including Crohn’s disease and ulcerative colitis, as well as primary sclerosing cholangitis (PSC) [5–6].
The huMST1(2) mouse was generated using gene-editing technology to replace the endogenous mouse Mst1 gene sequence from the 5′ untranslated region (5′UTR) to the 3′ untranslated region (3′UTR) with the corresponding human MST1 gene sequence spanning the 5′UTR to the 3′UTR. This model is primarily applicable to mechanistic studies of inflammatory bowel disease (IBD), primary sclerosing cholangitis, macrophage-associated inflammation, and immune regulation, as well as the exploration of therapeutic strategies targeting the MST1/MSP–RON pathway. It can be used to evaluate the potential effects of related drugs in inflammatory and immune diseases.
Reference
Yoshimura, T., Yuhki, N., Wang, M H., Skeel, A., & Leonard, E J.. (1993). Cloning, sequencing, and expression of human macrophage stimulating protein (MSP, MST1) confirms MSP as a member of the family of kringle proteins and locates the MSP gene on chromosome 3. The Journal of biological chemistry, 268(21), 15461-8.
Han, S., Stuart, L A., & Degen, S J.. (1991). Characterization of the DNF15S2 locus on human chromosome 3: identification of a gene coding for four kringle domains with homology to hepatocyte growth factor. Biochemistry, 30(40), 9768-80.
Skeel, A., Yoshimura, T., Showalter, S D., Tanaka, S., & Appella, E.. (1991). Macrophage stimulating protein: purification, partial amino acid sequence, and cellular activity. The Journal of experimental medicine, 173(5), 1227-34.
Sakamoto, O., Iwama, A., Amitani, R., Takehara, T., & Yamaguchi, N.. (1997). Role of macrophage-stimulating protein and its receptor, RON tyrosine kinase, in ciliary motility. The Journal of clinical investigation, 99(4), 701-9.
Goyette, P., Lefebvre, C., Ng, A., Brant, S R., & Cho, J H.. (2008). Gene-centric association mapping of chromosome 3p implicates MST1 in IBD pathogenesis. Mucosal immunology, 1(2), 131-8.
Melum, Espen., Franke, Andre., Schramm, Christoph., Weismüller, Tobias J., & Gotthardt, Daniel Nils.. (2010). Genome-wide association analysis in primary sclerosing cholangitis identifies two non-HLA susceptibility loci. Nature genetics, 43(1).
Strain Strategy
The sequence of the endogenous mouse Mst1 gene from the 5′UTR to the 3′UTR was replaced with that of the human MST1 gene from the 5′UTR to the 3′UTR. The KI region may affect the expression of mouse Mir7088, while its effect on mouse Rnf123 remains unclear.
Figure 1. Gene editing strategy of huMST1(2) mice.
Application Area
Mechanistic studies of inflammatory bowel disease (IBD), including Crohn’s disease and ulcerative colitis, primary sclerosing cholangitis (PSC), macrophage-associated inflammation, and immune regulation;
Exploration of therapeutic strategies targeting the MST1/MSP–RON pathway.
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.