Ckm-iCre-Tg Mouse
Product Name
Ckm-iCre-Tg Mouse
Product ID
C002148
Strain Name
C57BL/6Cya-Tg(Ckm-iCre)/Cya
Background
C57BL/6Cya
Examples of Expressing Tissues/Cells
Skeletal muscle, cardiac muscle, and other Ckm-positive cells
Status
Citations
0
When using this mouse strain in a publication, please cite “Ckm-iCre-Tg Mouse (Catalog C002148) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Standard products are sold as live mice derived from cryorecovery or expansion. Frozen materials are available upon request. Pricing and lead times depend on real-time inventory.
Gene Name
Ckm
Gene Alias
MCK, Ckmm, M-CK, CPK-M
NCBI ID
Chromosome
Chr 7 (Mouse)
MGI ID
Datasheet
Strain Description
The Ckm gene (creatine kinase, muscle) encodes muscle-type creatine kinase (CKM), a cellular energy metabolism enzyme primarily localized in the cytoplasm. Ckm is highly expressed in the skeletal muscle and myocardium of adult mice, and relatively high expression can also be detected in tissues such as the mammary gland. CKM catalyzes the reversible transfer of a phosphate group between phosphocreatine and ADP, promoting the rapid regeneration of ATP and maintaining the energy supply-demand balance during muscle contraction. Therefore, Ckm is an important gene for studying muscle energy metabolism and other muscle cell-related biological processes [1-2].
The Ckm-iCre-Tg mouse is a mouse model constructed by integrating the "Mouse Ckm promoter-Kozak-iCre-WPRE-BGH pA" cassette into the mouse genome via transgenic (TG) technology. iCre (improved Cre) is a codon-optimized form of Cre recombinase; in this document, "Cre recombinase" refers to iCre. When Ckm-iCre-Tg mice are crossed with mice containing loxP sites, the offspring are expected to undergo Cre recombinase-mediated sequence recombination between loxP sites in Ckm-positive cells.
Reference
Wallimann T, Tokarska-Schlattner M, Schlattner U. The creatine kinase system and pleiotropic effects of creatine. Amino Acids. 2011;40(5):1271-1296.
Kitzenberg D, Colgan SP, Glover LE. Creatine kinase in ischemic and inflammatory disorders. Clin Transl Med. 2016;5(1):31.
Strain Strategy
The "Mouse Ckm promoter-Kozak-iCre-WPRE-BGH pA" cassette was integrated into the mouse genome via transgenic (TG) technology.
Figure 1. Gene editing strategy of Ckm-iCre-Tg mice.
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