Adam11-3xV5 tag-P2A-iCreERT2-T2A-3xNLS-3xHA tag Mouse

Adam11 (a disintegrin and metallopeptidase domain 11) encodes ADAM11, a member of the ADAM family and a type I transmembrane protein. Because key residues of the catalytic motif are absent, ADAM11 is generally considered to lack metalloproteinase activity; in the central nervous system it has been implicated in neuronal adhesion and in the localization of presynaptic proteins ^[1,2]. Adam11 has been reported to be expressed in neurons throughout the central nervous system, with relatively high levels in the cerebellar cortex ^[1]; at the protein level, ADAM11 is enriched at cerebellar basket cell axon terminals (pinceau) and is weakly expressed in Purkinje cells, with expression also detected in the cerebral cortex, brain stem nuclei and spinal cord ^[2]. Expression has additionally been detected in the retina, peripheral nervous system ganglia, liver and testis, and at lower levels in the kidney ^[1].

Adam11-3xV5 tag-P2A-iCreERT2-T2A-3xNLS-3xHA tag mice were generated by gene editing, in which the stop codon of the endogenous mouse Adam11 gene was replaced with the "3xV5 tag-P2A-iCreERT2-T2A-3xNLS-3xHA tag" cassette. In the absence of tamoxifen treatment, the iCreERT2 recombinase is mainly localized in the cytoplasm; after tamoxifen treatment, the iCreERT2 recombinase enters the nucleus and exerts its recombination function. When Adam11-3xV5 tag-P2A-iCreERT2-T2A-3xNLS-3xHA tag mice are crossed with mice carrying loxP sites, the offspring are expected to undergo Cre recombinase-mediated recombination between loxP sites in Adam11-positive cells after tamoxifen induction.