Angptl4-P2A-DreERT2 Mouse
Product Name
Angptl4-P2A-DreERT2 Mouse
Product ID
C002210
Strain Name
C57BL/6Cya-Angptl4em1(P2A-DreERT2)/Cya
Background
C57BL/6Cya
Examples of Expressing Tissues/Cells
Adipocytes, hepatocytes, and other Angptl4-positive cells.
Status
When using this mouse strain in a publication, please cite “Angptl4-P2A-DreERT2 Mouse (Catalog C002210) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Standard products are sold as live mice derived from cryorecovery or expansion. Frozen materials are available upon request. Pricing and lead times depend on real-time inventory.
Gene Name
Angptl4
Gene Alias
Arp4, Bk89, Fiaf, Ng27, Pgar, Hfarp, Pgarg, Pp1158
NCBI ID
Chromosome
Chr 17 (Mouse)
MGI ID
Datasheet
Strain Description
Angptl4 (angiopoietin-like 4) encodes angiopoietin-like protein 4 (ANGPTL4), a secreted glycoprotein of the angiopoietin-like family that inhibits lipoprotein lipase (LPL) activity and participates in the partitioning and metabolic regulation of plasma triglycerides [1]. Angptl4 is a target gene of peroxisome proliferator-activated receptors (PPARs); it is expressed in adipose tissue and can be induced by fasting and by PPAR agonists [2]. The gene has been reported to be expressed relatively abundantly in adipocytes and hepatocytes, and liver-derived ANGPTL4 contributes to the partitioning of circulating triglycerides among tissues [1,3].
Angptl4-P2A-DreERT2 mice were generated by gene editing, in which the stop codon of the endogenous mouse Angptl4 gene was replaced with the "P2A-DreERT2" cassette. In the absence of tamoxifen treatment, the DreERT2 recombinase is mainly localized in the cytoplasm; after tamoxifen treatment, the DreERT2 recombinase enters the nucleus and exerts its recombination function. When Angptl4-P2A-DreERT2 mice are crossed with mice carrying rox sites, the offspring are expected to undergo Dre recombinase-mediated recombination between rox sites in Angptl4-positive cells after tamoxifen induction.
Reference
Dijk W, Kersten S. Regulation of lipoprotein lipase by Angptl4. Trends in Endocrinology and Metabolism. 2014;25(3):146-155. doi:10.1016/j.tem.2013.12.005.
Kersten S, Mandard S, Tan NS, Escher P, Metzger D, Chambon P, Gonzalez FJ, Desvergne B, Wahli W. Characterization of the fasting-induced adipose factor FIAF, a novel peroxisome proliferator-activated receptor target gene. Journal of Biological Chemistry. 2000;275(37):28488-28493. doi:10.1074/jbc.m004029200.
Singh AK, Chaube B, Zhang X, Sun J, Citrin KM, Canfrán-Duque A, Aryal B, Rotllan N, Varela L, Lee RG, Horvath TL, Price N, Suárez Y, Fernandez-Hernando C. Hepatocyte-specific suppression of ANGPTL4 improves obesity-associated diabetes and mitigates atherosclerosis in mice. Journal of Clinical Investigation. 2021;131(14):e140989.
Strain Strategy
The stop codon of the endogenous mouse Angptl4 gene was replaced with the "P2A-DreERT2" cassette.
Figure 1. Gene editing strategy of Angptl4-P2A-DreERT2 mice.
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