Itgax-DreERT2 Mouse
Product Name
Itgax-DreERT2 Mouse
Product ID
C002272
Strain Name
C57BL/6Cya-Itgaxem2(DreERT2)/Cya
Background
C57BL/6Cya
Examples of Expressing Tissues/Cells
Dendritic cells, monocytes, macrophages, granulocytes, and Itgax-positive cells
Status
Citations
0
When using this mouse strain in a publication, please cite “Itgax-DreERT2 Mouse (Catalog C002272) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Standard products are sold as live mice derived from cryorecovery or expansion. Frozen materials are available upon request. Pricing and lead times depend on real-time inventory.
Gene Name
Itgax
Gene Alias
Cr4, N418, Cd11c
NCBI ID
Chromosome
Chr 7 (Mouse)
MGI ID
Datasheet
Strain Description
The Itgax (integrin alpha X) gene encodes integrin αX (CD11c), a member of the leukocyte β2 integrin family. It non-covalently binds to the β2 subunit (CD18) to form the heterodimer CD11c/CD18 (αXβ2), also known as complement receptor 4 (CR4), which can recognize ligands such as iC3b and mediate cell adhesion and phagocytosis [1]. CD11c is a commonly used surface marker for dendritic cells; its expression level is relatively high on conventional dendritic cells, while relatively low on plasmacytoid dendritic cells. Among macrophages, alveolar macrophages are CD11c positive, whereas interstitial macrophages in the lung show limited expression [2-3]; in addition, neutrophils, some B cells and T cells, and a subset of NK cells also express CD11c [1].
Itgax-DreERT2 mice were generated by gene editing technology, in which the "Kozak-DreERT2-WPRE-BGH pA" cassette was inserted upstream of the start codon of the endogenous mouse Itgax gene. In the absence of tamoxifen treatment, DreERT2 recombinase is mainly localized in the cytoplasm; after tamoxifen treatment, DreERT2 recombinase enters the nucleus and exerts its recombination activity. When Itgax-DreERT2 mice are crossed with mice containing rox sites, after tamoxifen induction, the offspring are expected to undergo Dre recombinase-mediated sequence recombination between rox sites in Itgax-positive cells.
Reference
Hou L, Koutsogiannaki S, Yuki K. Multifaceted, unique role of CD11c in leukocyte biology. Front Immunol. 2025;16:1556992. doi:10.3389/fimmu.2025.1556992.
Guilliams M, Lambrecht BN, Hammad H. Division of labor between lung dendritic cells and macrophages in the defense against pulmonary infections. Mucosal Immunol. 2013;6(3):464–473. doi:10.1038/mi.2013.14.
Misharin AV, Morales-Nebreda L, Mutlu GM, Budinger GRS, Perlman H. Flow cytometric analysis of macrophages and dendritic cell subsets in the mouse lung. Am J Respir Cell Mol Biol. 2013;49(4):503–510. doi:10.1165/rcmb.2013-0086MA.
Strain Strategy
The "Kozak-DreERT2-WPRE-BGH pA" cassette was inserted upstream of the start codon of the endogenous mouse Itgax gene. This strategy disrupts the normal expression and function of the Itgax gene in mice.
Figure 1. Gene editing strategy of Itgax-DreERT2 mice.
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