Ncr1-IRES-CreERT2 Mouse

The Ncr1 gene encodes natural cytotoxicity receptor 1 (NCR1, also known as NKp46), a type I transmembrane glycoprotein belonging to the immunoglobulin superfamily. In mice, Ncr1 is mainly expressed in natural killer (NK) cells, and also in some innate lymphoid cells (ILCs), including ILC1 and certain ILC3 subsets. NCR1 is an important immune-activating receptor that recognizes pathogen-associated ligands such as viral hemagglutinin, and promotes NK cell activation and cytotoxic responses through cooperation with signaling adaptor proteins such as CD3ζ or the Fc receptor common γ chain, participating in antiviral immunity and tumor immune surveillance ^[1-2].

Ncr1-IRES-CreERT2 mice were generated by gene editing, in which the "IRES-CreERT2" cassette was inserted downstream of the stop codon of the endogenous mouse Ncr1 gene. In the absence of tamoxifen treatment, the CreERT2 recombinase is mainly localized in the cytoplasm; after tamoxifen treatment, the CreERT2 recombinase enters the nucleus and exerts its recombination function. When Ncr1-IRES-CreERT2 mice are crossed with mice carrying loxP sites, the offspring are expected to undergo Cre recombinase-mediated recombination between loxP sites in Ncr1-positive cells after tamoxifen induction.