H11-UBC-PSD95_GFP Mouse
Product Name
H11-UBC-PSD95_GFP Mouse
Product ID
C002307
Strain Name
C57BL/6Cya-Igs2em1(UBC-PSD95_GFP)/Cya
Background
C57BL/6Cya
Examples of Expressing Tissues/Cells
Postsynaptic density (PSD) in neurons
Status
When using this mouse strain in a publication, please cite “H11-UBC-PSD95_GFP Mouse (Catalog C002307) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Standard products are sold as live mice derived from cryorecovery or expansion. Frozen materials are available upon request. Pricing and lead times depend on real-time inventory.
Gene Name
Dlg4
Gene Alias
Dlgh4, PSD95, SAP90, PSD-95, SAP90A
NCBI ID
Chromosome
Chr 11 (Mouse)
MGI ID
Datasheet
Strain Description
Dlg4 (discs large MAGUK scaffold protein 4) gene encodes the postsynaptic density protein PSD-95 (postsynaptic density protein 95, also known as SAP-90), which belongs to the membrane-associated guanylate kinase (MAGUK) family and contains three PDZ domains, one SH3 domain, and one guanylate kinase-like (GK) domain. PSD-95 is mainly enriched in the postsynaptic density (PSD) of excitatory synapses in neurons. By organizing and stabilizing synaptic proteins such as NMDA receptors, AMPA receptors, and Shaker-type potassium channels, it participates in the organization and maturation of excitatory synapses and the regulation of synaptic plasticity [1-2]. The PSD-95-GFP fusion protein has been used to label excitatory postsynaptic sites [3].
In this model, the "UBC promoter-Kozak-PSD95_GFP-polyA" cassette was inserted into the H11 locus (intergenic region between the Eif4enif1 and Drg1 genes on mouse chromosome 11). This model can be used for studies on the postsynaptic density (PSD).
Reference
Sheng M, Kim E. The postsynaptic organization of synapses. Cold Spring Harb Perspect Biol. 2011;3(12):a005678.
El-Husseini AE, Schnell E, Chetkovich DM, Nicoll RA, Bredt DS. PSD-95 involvement in maturation of excitatory synapses. Science. 2000;290(5495):1364-1368.
Okabe S, Kim HD, Miwa A, Kuriu T, Okado H. Continual remodeling of postsynaptic density and its regulation by synaptic activity. Nat Neurosci. 1999;2(9):804-811.
Strain Strategy
The "UBC promoter-Kozak-PSD95_GFP-polyA" cassette was inserted into the H11 locus (intergenic region between the Eif4enif1 and Drg1 genes on mouse chromosome 11).
Figure 1. Gene editing strategy of H11-UBC-PSD95_GFP mice.
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