Trbc1-P2A-CreERT2-T2A-EGFP Mouse
Product Name
Trbc1-P2A-CreERT2-T2A-EGFP Mouse
Product ID
C002344
Strain Name
C57BL/6Cya-Trbc1em1(P2A-CreERT2-T2A-EGFP)/Cya
Background
C57BL/6Cya
Examples of Expressing Tissues/Cells
αβ T cells using Cβ1, and other Trbc1-positive cells.
Status
When using this mouse strain in a publication, please cite “Trbc1-P2A-CreERT2-T2A-EGFP Mouse (Catalog C002344) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Standard products are sold as live mice derived from cryorecovery or expansion. Frozen materials are available upon request. Pricing and lead times depend on real-time inventory.
Gene Name
Trbc1
Gene Alias
Gm16802, Tcrb-C1
NCBI ID
Chromosome
Chr 6 (Mouse)
MGI ID
Datasheet
Strain Description
TRBC1 (T cell receptor beta constant 1) encodes the T cell receptor beta chain constant region 1 and is a component of the αβ T cell receptor (TCR). During αβ T cell development, TCRβ gene rearrangement involves mutually exclusive selection between the two constant regions, Trbc1 and Trbc2. Consequently, the mature T cell population typically includes both TRBC1-positive and TRBC1-negative cells [1-2]. Based on this feature, TRBC1 has been widely used for T cell clonality analysis and can be combined with flow cytometry to assess the clonal composition of T cell populations [1-2]. In recent years, TRBC1 has also been explored as a molecular target for targeted therapy of T cell malignancies, providing new research directions for T cell-related disease studies and immunotherapy [3].
The Trbc1-P2A-CreERT2-T2A-EGFP mouse was constructed using gene editing technology by replacing the endogenous mouse Trbc1 gene stop codon with the "P2A-CreERT2-T2A-EGFP" cassette. In the absence of Tamoxifen treatment, CreERT2 recombinase is primarily localized in the cytoplasm. Upon Tamoxifen induction, CreERT2 recombinase can enter the nucleus and exert its recombination activity. When Trbc1-P2A-CreERT2-T2A-EGFP mice are crossed with mice carrying loxP sites, the offspring, after Tamoxifen induction, are expected to achieve CreERT2 recombinase-mediated sequence recombination between loxP sites in Trbc1-positive cells.
Reference
Horna P, Shi M, Olteanu H, Johansson U. Emerging Role of T-cell Receptor Constant β Chain-1 (TRBC1) Expression in the Flow Cytometric Diagnosis of T-cell Malignancies. Int J Mol Sci. 2021 Feb 12;22(4):1817.
Devitt KA, Kern W, Li W, Wang X, Wong AJ, Furtado FM, Oak JS, Illingworth A. TRBC1 in flow cytometry: Assay development, validation, and reporting considerations. Cytometry B Clin Cytom. 2024 May;106(3):192-202.
Ferrari M, Righi M, Baldan V, Wawrzyniecka P, Bulek A, Kinna A, Ma B, Bughda R, Akbar Z, Srivastava S, Gannon I, Robson M, Sillibourne J, Jha R, El-Kholy M, Amin OM, Kokalaki E, Banani MA, Hussain R, Day W, Lim WC, Ghongane P, Hopkins JR, Jungherz D, Herling M, Welin M, Surade S, Dyson M, McCafferty J, Logan D, Cordoba S, Thomas S, Sewell A, Maciocia P, Onuoha S, Pule M. Structure-guided engineering of immunotherapies targeting TRBC1 and TRBC2 in T cell malignancies. Nat Commun. 2024 Feb 21;15(1):1583.
Strain Strategy
The stop codon of mouse Trbc1 gene was replaced with "P2A-CreERT2-T2A-EGFP" cassette.
Figure 1. Gene editing strategy of Trbc1-P2A-CreERT2-T2A-EGFP mice.
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