SD-H11-hREN Rat
Product Name
SD-H11-hREN Rat
Product ID
CR009
Strain Name
SD-Igs2em1(hREN)/Cya
Backgroud
SD
Status
When using this mouse strain in a publication, please cite “SD-H11-hREN Rat (Catalog CR009) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Gene Name
REN
Gene Alias
RTD, HNFJ2, ADTKD4
NCBI ID
Chromosome
Chr 1 (Human)
MGI ID
Datasheet
Strain Description
The REN gene encodes renin, a crucial aspartyl protease and the rate-limiting enzyme of the renin-angiotensin system (RAS), which is fundamental for regulating blood pressure and electrolyte balance [1]. Primarily expressed in the juxtaglomerular cells of the kidneys, renin is secreted into the bloodstream where it functions to cleave angiotensinogen, produced by the liver, into angiotensin I [2]. This initiates a cascade leading to the formation of angiotensin II, a potent vasoconstrictor that also stimulates aldosterone release from the adrenal glands, ultimately increasing blood pressure and sodium retention [3]. Mutations in the REN gene have been linked to several renal disorders, including familial juvenile hyperuricemic nephropathy type II, familial hyperreninemia, and renal tubular dysgenesis [4].
SD-H11-hREN rats are a humanized model generated using gene editing technology, in which the “3 kb of 5’- flanking sequence-Human REN DNA-1.2 kb of 3'-flanking sequence” cassette was inserted into H11 locus. Homozygous SD-H11-hREN rats are viable and fertile. This model can be used for research on the regulation of blood pressure, body fluid, and electrolyte homeostasis, as well as for studying the pathogenic mechanisms and drug development for various hereditary kidney diseases such as renal tubular dysgenesis and familial hyperreninemia.
Reference
Bader M, Steckelings UM, Alenina N, Santos RAS, Ferrario CM. Alternative Renin-Angiotensin System. Hypertension. 2024 May;81(5):964-976.
Vargas Vargas RA, Varela Millán JM, Fajardo Bonilla E. Renin-angiotensin system: Basic and clinical aspects-A general perspective. Endocrinol Diabetes Nutr (Engl Ed). 2022 Jan;69(1):52-62.
Yamaguchi H, Gomez RA, Sequeira-Lopez MLS. Renin Cells, From Vascular Development to Blood Pressure Sensing. Hypertension. 2023 Aug;80(8):1580-1589.
Kobori H, Nangaku M, Navar LG, Nishiyama A. The intrarenal renin-angiotensin system: from physiology to the pathobiology of hypertension and kidney disease. Pharmacol Rev. 2007 Sep;59(3):251-87.
Strain Strategy
The “3 kb of 5’- flanking sequence-Human REN DNA-1.2 kb of 3'-flanking sequence” cassette was inserted into H11 locus.
Figure 1. Gene editing strategy of SD-H11-hREN rats.
Application Area
REN-targeted drug screening, development, and evaluation；
Research on homeostatic regulation of blood pressure, body fluids, and electrolytes；
Research on the pathological mechanisms and therapeutic approaches of various hereditary kidney diseases such as renal tubular dysgenesis and familial hyperreninemia.
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