This strain carries an EIIA gene promoter-driven Improved Cre (iCre) recombinase gene expression element insertion in the H11 safe harbor to drive the systemic expression of Cre recombinase. When crossed with mice containing the loxP site-flanked sequence, Cre recombinase-mediated recombination resulted in the deletion of the flanked sequence in the early embryo of offspring. The recombination occurs in systemic tissues and can transmit genetic changes to the germ cells of the offspring in this strain. The homozygous H11-EIIA-iCre mice are viable and fertile.