HLA-A24 (A24) is a human leukocyte antigen of the HLA-A serotype group, with its alpha chain encoded by the HLA-A*24 allele and its beta chain encoded by the B2M gene. The H11-hB2M&HLA-A24 mouse model is constructed by integrating the chimeric H2-K1 HLA-A24 gene into the H11 safe harbor locus of mice using gene editing technology. This humanized model can be used to identify viral peptides that express HLA-A24 binding sequences.