TG-CAG-mito-mNeonGreen Mouse
Product Name
TG-CAG-mito-mNeonGreen Mouse
Product ID
I001183
Strain Name
C57BL/6JCya-Tg(CAG-mito-mNeonGreen)/Cya
Backgroud
C57BL/6JCya
Status
When using this mouse strain in a publication, please cite “TG-CAG-mito-mNeonGreen Mouse (Catalog I001183) were purchased from Cyagen.”
Product Type
Age
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Strain Description
This strain is a fluorescently labeled model carrying a constitutively fluorescent green/yellow protein (mNeonGreen) reporter gene expression element, with mNeonGreen protein expression regulated by the CAG promoter. The CAG promoter is constructed by modifying and integrating the chicken β-actin gene promoter, the cytomegalovirus (CMV) early enhancer, the rabbit β-actin gene splice acceptor, and the bovine globin polyA signal. It is renowned for its strong and stable real-time gene expression in various mammalian cells, making it a popular choice for driving gene expression in transgenic models. The mNeonGreen protein is a monomeric green/yellow fluorescent protein derived from the lanthanoid bronchitis virus, and it can be used as a fusion tag for conventional imaging and stochastic single-molecule super-resolution imaging. It is also an excellent fluorescence resonance energy transfer (FRET) acceptor for cyan fluorescent protein [1]. This strain is constructed by integrating the CAG-mito-mNeonGreen gene expression cassette into the mouse genome using transgenic technology, resulting in the specific expression of the mNeonGreen protein in mitochondria.
Reference
Shaner NC, Lambert GG, Chammas A, Ni Y, Cranfill PJ, Baird MA, Sell BR, Allen JR, Day RN, Israelsson M, Davidson MW, Wang J. A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum. Nat Methods. 2013 May;10(5):407-9.
Strain Strategy
The CAG-chimeric intron-mito-mNeonGreen-poly A gene expression cassette was integrated into the mouse genome using transgenic technology.
Figure 1. Gene editing strategy of TG-CAG-mito-mNeonGreen mice.
Application Area
Research on the functions, localization, and dynamics of mitochondria.
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