The Rosa26-hHRAS mouse model is a humanized model constructed by integrating a human HRAS gene with the c.450+180A>G mutation into the Rosa26 safe harbor site in mice using gene editing technology. The introduction of the c.450+180A>G mutation significantly enhances HRAS gene expression. This model can be used for rapid in vivo testing of the carcinogenicity of genotoxic and non-genotoxic compounds, studying the impact of HRAS oncogene point mutations on tumorigenesis and development, and developing tumor prevention or suppression therapies.