C57BL/6JCya-Zzz3em1flox/Cya
Common Name:
Zzz3-flox
Product ID:
S-CKO-00723
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Zzz3-flox
Strain ID
CKOCMP-108946-Zzz3-B6J-VA
Gene Name
Product ID
S-CKO-00723
Gene Alias
3110065C23Rik; 6430567E01Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Zzz3em1flox/Cya mice (Catalog S-CKO-00723) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000106100
NCBI RefSeq
NM_001080755
Target Region
Exon 5~7
Size of Effective Region
~3.0 kb
Detailed Document
Overview of Gene Research
Zzz3, also known as zinc finger ZZ-type containing 3, is involved in multiple biological processes. It is a subunit of the Ada-two-A-containing (ATAC) histone acetyltransferase complex, and its ZZ-type zinc finger domain acts as a histone H3 reader, modulating ATAC-mediated histone acetylation and gene activation [3]. It also plays a role in pathways related to cell proliferation, ribosome biogenesis, and translation, and is associated with processes in human embryonic stem cells (ESCs) homeostasis and immune-related interactions in diffuse large B-cell lymphoma (DLBCL) [1,2].
In human ESCs, knockdown of Zzz3 negatively impacts ribosome biogenesis, translation, and mTOR signaling, leading to a significant reduction in cell proliferation without affecting pluripotency, suggesting ESCs enter a "dormant-like" state [1]. In DLBCL, Zzz3 upregulates the transcription of CD70 by binding to its super-enhancer, and DLBCL cells inhibit the proliferation and killing of infiltrating NK cells by regulating the Zzz3/CD70 axis [2]. A bioinformatics analysis also suggested that Zzz3 may be related to cell proliferation in non-union skeletal fractures [4].
In conclusion, Zzz3 is essential in processes like histone acetylation, gene activation, and cell proliferation. Its study in models such as human ESCs and DLBCL cell-NK cell interactions has provided insights into stem cell homeostasis and cancer-immune cell relationships, respectively. Understanding Zzz3's function may offer new perspectives on these biological processes and potentially lead to novel therapeutic strategies for related diseases.
References：
1. Lo Conte, Michela, Lucchino, Valeria, Scalise, Stefania, Cuda, Giovanni, Parrotta, Elvira Immacolata. 2024. Unraveling the impact of ZZZ3 on the mTOR/ribosome pathway in human embryonic stem cells homeostasis. In Stem cell reports, 19, 729-743. doi:10.1016/j.stemcr.2024.04.002. https://pubmed.ncbi.nlm.nih.gov/38701777/
2. Li, Xi, Cui, Juya, Wang, Liao, Cao, Caihong, Liu, Hu. 2024. Integrated multi-omics profiling reveals the ZZZ3/CD70 axis is a super-enhancer-driven regulator of diffuse large B-cell lymphoma cell-natural killer cell interactions. In Experimental biology and medicine (Maywood, N.J.), 249, 10155. doi:10.3389/ebm.2024.10155. https://pubmed.ncbi.nlm.nih.gov/39376717/
3. Mi, Wenyi, Zhang, Yi, Lyu, Jie, Kutateladze, Tatiana G, Shi, Xiaobing. 2018. The ZZ-type zinc finger of ZZZ3 modulates the ATAC complex-mediated histone acetylation and gene activation. In Nature communications, 9, 3759. doi:10.1038/s41467-018-06247-5. https://pubmed.ncbi.nlm.nih.gov/30217978/
4. Zhang, Jun. . Bioinformatics analysis of novel transcription factors and related differentially regulated modules in non-union skeletal fractures. In Journal of back and musculoskeletal rehabilitation, 31, 623-628. doi:10.3233/BMR-169596. https://pubmed.ncbi.nlm.nih.gov/29578472/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen