C57BL/6JCya-Ehmt2em1flox/Cya
Common Name:
Ehmt2-flox
Product ID:
S-CKO-00835
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ehmt2-flox
Strain ID
CKOCMP-110147-Ehmt2-B6J-VA
Gene Name
Product ID
S-CKO-00835
Gene Alias
Bat8; D17Ertd710e; G9a; KMT1C; NG36
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ehmt2em1flox/Cya mice (Catalog S-CKO-00835) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000013931
NCBI RefSeq
NM_145830
Target Region
Exon 7~20
Size of Effective Region
~4.2 kb
Detailed Document
Overview of Gene Research
Ehmt2, also known as euchromatic histone lysine methyltransferase 2 or G9a, is a SET domain-containing histone lysine methyltransferase. It is mainly responsible for mono, di and tri methylation of histone H3K9 in euchromatin, playing a role in transcriptional regulation, genome methylation and other processes. It is an essential developmental gene involved in embryonic development, establishment of proviral silencing in ES cells, and is associated with many biological functions and diseases [3,4].
In microsatellite stability (MSS) colorectal cancer, EHMT2 inhibition can transform the immunosuppressive microenvironment into an immunomodulatory one, leading to T-cell-mediated cytotoxicity activation and improved responsiveness to anti-PD1 treatment. Mechanistically, EHMT2 forms a cotranscriptional silencing complex with CHD4 to repress galectin-7 expression, and galectin-7 up-regulation upon EHMT2 inhibition can convert a "cold" tumor environment into a T-cell-inflamed one [1].
In non-small cell lung cancer, EHMT2-mediated transcriptional reprogramming drives neuroendocrine transformation, increasing methylation of the SFRP1 promoter region to reduce SFRP1 expression, followed by activation of the WNT/β-catenin pathway. Suppression of EHMT2 with selective inhibitors can restore the sensitivity of neuroendocrine transformation cell lines to erlotinib [2].
In GNAQ/11 -mutant uveal melanoma, EHMT2 promotes tumorigenesis via ARHGAP29-mediated RhoA pathway. Inhibition of EHMT2 expression or activity significantly reduces the proliferation and migration capacity of cancer cells [5].
In conclusion, Ehmt2 is a crucial epigenetic regulator involved in multiple disease-related biological processes. Its functions in cancers such as MSS colorectal cancer, non-small cell lung cancer, and uveal melanoma are revealed through various functional studies. The research on Ehmt2 provides potential therapeutic targets for these diseases, emphasizing the importance of understanding its role in disease mechanisms for developing effective treatment strategies.
References：
1. Sun, Lei, Liu, Ruonian, Wu, Zong-Jian, Kuang, Dong-Ming, Wan, Guohui. 2023. Galectin-7 Induction by EHMT2 Inhibition Enhances Immunity in Microsatellite Stability Colorectal Cancer. In Gastroenterology, 166, 466-482. doi:10.1053/j.gastro.2023.11.294. https://pubmed.ncbi.nlm.nih.gov/38065340/
2. Yang, Cheng, Ma, Shuxiang, Zhang, Jie, Wang, Qiming, Wang, Lihui. 2024. EHMT2-mediated transcriptional reprogramming drives neuroendocrine transformation in non-small cell lung cancer. In Proceedings of the National Academy of Sciences of the United States of America, 121, e2317790121. doi:10.1073/pnas.2317790121. https://pubmed.ncbi.nlm.nih.gov/38814866/
3. Souza, Barbara Kunzler, Freire, Natalia Hogetop, Jaeger, Mariane, Brunetto, André T, Roesler, Rafael. 2021. EHMT2/G9a as an Epigenetic Target in Pediatric and Adult Brain Tumors. In International journal of molecular sciences, 22, . doi:10.3390/ijms222011292. https://pubmed.ncbi.nlm.nih.gov/34681949/
4. Jan, Suraya, Dar, Mohd Ishaq, Wani, Rubiada, Lateef, Sammar, Syed, Sajad Hussain. 2020. Targeting EHMT2/ G9a for cancer therapy: Progress and perspective. In European journal of pharmacology, 893, 173827. doi:10.1016/j.ejphar.2020.173827. https://pubmed.ncbi.nlm.nih.gov/33347828/
5. Li, Yongyun, Zhu, Tianyu, Yang, Jie, Zhang, Jianming, Fan, Xianqun. 2023. EHMT2 promotes tumorigenesis in GNAQ/11-mutant uveal melanoma via ARHGAP29-mediated RhoA pathway. In Acta pharmaceutica Sinica. B, 14, 1187-1203. doi:10.1016/j.apsb.2023.12.002. https://pubmed.ncbi.nlm.nih.gov/38486999/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen