C57BL/6JCya-Gpr18em1flox/Cya
Common Name
Gpr18-flox
Product ID
S-CKO-00837
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-110168-Gpr18-B6J-VA
Status
When using this mouse strain in a publication, please cite “Gpr18-flox Mouse (Catalog S-CKO-00837) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Gpr18-flox
Strain ID
CKOCMP-110168-Gpr18-B6J-VA
Gene Name
Product ID
S-CKO-00837
Gene Alias
--
Background
C57BL/6JCya
Gene Full Name
G protein-coupled receptor 18
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 14 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000055475
NCBI Transcript ID
NM_182806
Target Region
Exon 2
Size of Effective Region
~2.5 kb
Overview of Gene Research
Gpr18, a G-protein-coupled receptor, belongs to the orphan class A family of the GPCR superfamily [2]. It has been suggested to be related to the endocannabinoid system, despite low sequence homology with cannabinoid receptors CB1R and CB2R [2]. Gpr18 may play roles in the migration and proliferation of cancer cells, macrophages, and other inflammation-associated immune cells, making it a potential target for inflammation, cancer, and analgesia therapy [1]. It is also involved in the resolution of inflammation, as it serves as the receptor for resolvin D2 (RvD2), a specialized pro-resolving mediator, and the RvD2/Gpr18 axis has a protective role in cardiovascular and metabolic diseases (CVMDs) such as atherosclerosis, hypertension, ischaemia-reperfusion, and diabetes [3,4,5].
In models of Duchenne muscular dystrophy, treatment with the Gpr18 agonist PSB-KD107 reduced inflammation, enhanced myogenesis, and improved muscle function, suggesting a therapeutic potential for this disorder [6]. Verbenalin, a component of Verbena in Xuanfeibaidu (XFBD) granules, alleviates acute lung injury induced by sepsis and IgG immune complex through direct binding to the Gpr18 receptor, inhibiting inflammatory signaling pathways and macrophage pyroptosis [7].
In conclusion, Gpr18 is a significant G-protein-coupled receptor involved in multiple biological processes, especially those related to inflammation and disease resolution. Studies using agonists in in vivo models like Duchenne muscular dystrophy and acute lung injury models have demonstrated its potential as a therapeutic target, highlighting its importance in understanding disease mechanisms and developing new treatments for related diseases.
References：
1. Zhang, Linjie, Fang, Yiwen, Hang, Sijing, Sheng, Ruilong, Guo, Ruihua. . GPR18 and GPR55-related Ligands Serving as Antagonists or Agonists: Current Situation, Challenges and Perspectives. In Medicinal chemistry (Shariqah (United Arab Emirates)), 19, 838-847. doi:10.2174/1573406419666230406095220. https://pubmed.ncbi.nlm.nih.gov/37038675/
2. Morales, Paula, Lago-Fernandez, Ana, Hurst, Dow P, Abood, Mary E, Jagerovic, Nadine. 2020. Therapeutic Exploitation of GPR18: Beyond the Cannabinoids? In Journal of medicinal chemistry, 63, 14216-14227. doi:10.1021/acs.jmedchem.0c00926. https://pubmed.ncbi.nlm.nih.gov/32914978/
3. Zhao, Mengmeng, Zheng, Zihui, Yin, Zheng, Wan, Jun, Wang, Menglong. 2023. Resolvin D2 and its receptor GPR18 in cardiovascular and metabolic diseases: A promising biomarker and therapeutic target. In Pharmacological research, 195, 106832. doi:10.1016/j.phrs.2023.106832. https://pubmed.ncbi.nlm.nih.gov/37364787/
4. Honkisz-Orzechowska, Ewelina, Łażewska, Dorota, Baran, Grzegorz, Kieć-Kononowicz, Katarzyna. 2024. Uncovering the Power of GPR18 Signalling: How RvD2 and Other Ligands Could Have the Potential to Modulate and Resolve Inflammation in Various Health Disorders. In Molecules (Basel, Switzerland), 29, . doi:10.3390/molecules29061258. https://pubmed.ncbi.nlm.nih.gov/38542895/
5. Spite, Matthew, Fredman, Gabrielle. 2023. Insights into the role of the resolvin D2-GPR18 signaling axis in cardiovascular physiology and disease. In Advances in pharmacology (San Diego, Calif.), 97, 257-281. doi:10.1016/bs.apha.2022.12.005. https://pubmed.ncbi.nlm.nih.gov/37236761/
6. Dort, Junio, Orfi, Zakaria, Fiscaletti, Melissa, Campeau, Philippe M, Dumont, Nicolas A. 2023. Gpr18 agonist dampens inflammation, enhances myogenesis, and restores muscle function in models of Duchenne muscular dystrophy. In Frontiers in cell and developmental biology, 11, 1187253. doi:10.3389/fcell.2023.1187253. https://pubmed.ncbi.nlm.nih.gov/37645248/
7. Yang, Lei, Liu, Tianyu, Zhuo, Yuzhen, Lin, Jianping, Wang, Ximo. 2023. Verbenalin alleviates acute lung injury induced by sepsis and IgG immune complex through GPR18 receptor. In Cellular signalling, 109, 110768. doi:10.1016/j.cellsig.2023.110768. https://pubmed.ncbi.nlm.nih.gov/37315751/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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