C57BL/6JCya-Aldh1a1em1flox/Cya
Common Name:
Aldh1a1-flox
Product ID:
S-CKO-01133
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Aldh1a1-flox
Strain ID
CKOCMP-11668-Aldh1a1-B6J-VA
Gene Name
Product ID
S-CKO-01133
Gene Alias
ALDH-E1; ALHDII; Ahd-2; Ahd2; Aldh1; Aldh1a2; E1; Raldh1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
19
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Aldh1a1em1flox/Cya mice (Catalog S-CKO-01133) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000087638
NCBI RefSeq
NM_013467
Target Region
Exon 4
Size of Effective Region
~2.1 kb
Detailed Document
Overview of Gene Research
Aldh1a1, short for aldehyde dehydrogenase 1 family member A1, is an enzyme in retinoic acid biosynthesis and redox balance [2]. It can metabolize reactive aldehydes to their corresponding carboxylic acid derivatives and plays important physiological and toxicological roles in areas like the CNS, metabolic disorders, and cancers [7]. The cytosolic Aldh1a1 can irreversibly convert retinal to retinoic acid, initiating RA signaling, which is crucial for self-renewal and epithelial-to-mesenchymal transition (EMT) [5].
In cancer research, genetic silencing of Aldh1a1 in in vitro, xenografted zebrafish and murine models has shown its significance. In breast cancer, Aldh1a1 enzymatic activity promotes breast tumor growth by decreasing intracellular pH, phosphorylating TAK1, activating NFκB signaling, and increasing GM-CSF secretion, leading to myeloid-derived suppressor cell expansion and immunosuppression. Inhibiting Aldh1a1 with disulfiram and gemcitabine cooperatively suppresses breast tumor growth [1]. In prostate cancer, Aldh1a1 positively regulates tumor cell survival in circulation, extravasation, and metastatic dissemination, and its gain in bone metastases is associated with high PLK3 expression [3]. In EGFR-mutant lung cancer, genetic repression of Aldh1a1 in cancer cells reduces brain metastasis [4]. In glioblastoma, overexpressing Aldh1a1 in U87 cells increases cell growth and invasion, and inhibiting AKT phosphorylation mitigates these effects [6].
In conclusion, Aldh1a1 is a key enzyme in retinoic acid biosynthesis and redox balance. Through gene-knockout or silencing in model organisms, its roles in cancer progression, metastasis, and immune-related processes have been revealed. Targeting Aldh1a1 shows potential as a therapeutic strategy in various cancers, highlighting its importance in understanding disease mechanisms and developing new treatments.
References:
1. Liu, Cuicui, Qiang, Jiankun, Deng, Qiaodan, Zhang, Lixing, Liu, Suling. 2021. ALDH1A1 Activity in Tumor-Initiating Cells Remodels Myeloid-Derived Suppressor Cells to Promote Breast Cancer Progression. In Cancer research, 81, 5919-5934. doi:10.1158/0008-5472.CAN-21-1337. https://pubmed.ncbi.nlm.nih.gov/34580061/
2. Bian, Yunyi, Shan, Guangyao, Bi, Guoshu, Fan, Hong, Zhan, Cheng. 2024. Targeting ALDH1A1 to enhance the efficacy of KRAS-targeted therapy through ferroptosis. In Redox biology, 77, 103361. doi:10.1016/j.redox.2024.103361. https://pubmed.ncbi.nlm.nih.gov/39317105/
3. Gorodetska, Ielizaveta, Offermann, Anne, Püschel, Jakob, Perner, Sven, Dubrovska, Anna. 2024. ALDH1A1 drives prostate cancer metastases and radioresistance by interplay with AR- and RAR-dependent transcription. In Theranostics, 14, 714-737. doi:10.7150/thno.88057. https://pubmed.ncbi.nlm.nih.gov/38169509/
4. Biswas, Anup Kumar, Han, Seoyoung, Tai, Yifan, Massague, Joan, Acharyya, Swarnali. . Targeting S100A9-ALDH1A1-Retinoic Acid Signaling to Suppress Brain Relapse in EGFR-Mutant Lung Cancer. In Cancer discovery, 12, 1002-1021. doi:10.1158/2159-8290.CD-21-0910. https://pubmed.ncbi.nlm.nih.gov/35078784/
5. Datta, Nandini, Vp, Snijesh, Parvathy, K, A S, Sneha, Maliekal, Tessy Thomas. 2024. ALDH1A1 as a marker for metastasis initiating cells: A mechanistic insight. In Experimental cell research, 442, 114213. doi:10.1016/j.yexcr.2024.114213. https://pubmed.ncbi.nlm.nih.gov/39173941/
6. Huang, Yu-Kai, Wang, Tzu-Ming, Chen, Chi-Yu, Pan, Yuan, Chang, Kun-Che. 2024. The role of ALDH1A1 in glioblastoma proliferation and invasion. In Chemico-biological interactions, 402, 111202. doi:10.1016/j.cbi.2024.111202. https://pubmed.ncbi.nlm.nih.gov/39128802/
7. Li, Bingyan, Yang, Kang, Liang, Dailin, Jiang, Cheng, Ma, Zonghui. 2020. Discovery and development of selective aldehyde dehydrogenase 1A1 (ALDH1A1) inhibitors. In European journal of medicinal chemistry, 209, 112940. doi:10.1016/j.ejmech.2020.112940. https://pubmed.ncbi.nlm.nih.gov/33328099/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen