C57BL/6NCya-Rab27aem1flox/Cya
Common Name:
Rab27a-flox
Product ID:
S-CKO-01320
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Rab27a-flox
Strain ID
CKOCMP-11891-Rab27a-B6N-VA
Gene Name
Product ID
S-CKO-01320
Gene Alias
2210402C08Rik; 2410003M20Rik; 4933437C11Rik; ash
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Rab27aem1flox/Cya mice (Catalog S-CKO-01320) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000184146
NCBI RefSeq
NM_001301230
Target Region
Exon 4
Size of Effective Region
~1.9 kb
Detailed Document
Overview of Gene Research
Rab27a, a small GTPase, is a crucial regulator in multiple membrane-trafficking processes. It exists in an 'active' GTP-bound and 'inactive' GDP-bound state, acting as a molecular switch. It is involved in pathways such as exosome secretion, insulin secretion, and vesicular trafficking in various cell types, which are vital for intercellular communication, metabolism, and immune response [3,4,7]. Genetic models, like KO mouse models, are valuable for studying its functions in vivo.
In exosome secretion, RNA interference (RNAi) screening in HeLa cells showed that Rab27a functions in multivesicular endosome (MVE) docking at the plasma membrane, and its silencing strongly increased MVE size [1]. In pancreatic β-cells, Rab27a regulates pre-exocytotic stages of insulin secretion via GTP-specific effectors, and its GDP-bound form interacts with GDP-specific effectors to control endocytosis, synchronizing with secretory membrane recycling [4]. In neurons and podocytes, knockdown of KIBRA, which stabilizes Rab27a, decreased exosome secretion, while overexpression increased it [2]. In colon cancer cells, RAB27A knockdown inhibited proliferation, clone formation, migration, and invasion, indicating its role as an onco-protein in colorectal cancer development [5]. In cerebral ischemic injury, Rab27a knockout mice had decreased brain exosome levels, increased infarct volume, and neurological deficit scores, while pre-infusion of exosomes from wild-type mice improved the situation [6].
In conclusion, Rab27a is essential for membrane-trafficking related biological functions such as exosome secretion, insulin secretion, and vesicular trafficking. Model-based research, especially Rab27a KO mouse models, has revealed its significance in diseases like colorectal cancer and cerebral ischemic injury, providing insights into disease mechanisms and potential therapeutic targets.
References:
1. Ostrowski, Matias, Carmo, Nuno B, Krumeich, Sophie, Moita, Luis F, Thery, Clotilde. 2009. Rab27a and Rab27b control different steps of the exosome secretion pathway. In Nature cell biology, 12, 19-30; sup pp 1-13. doi:10.1038/ncb2000. https://pubmed.ncbi.nlm.nih.gov/19966785/
2. Song, Lin, Tang, Shi, Han, Xiaolei, Wang, Yongxiang, Du, Yifeng. 2019. KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a. In Nature communications, 10, 1639. doi:10.1038/s41467-019-09720-x. https://pubmed.ncbi.nlm.nih.gov/30967557/
3. Kimura, Toshihide, Niki, Ichiro. 2010. Rab27a, actin and beta-cell endocytosis. In Endocrine journal, 58, 1-6. doi:. https://pubmed.ncbi.nlm.nih.gov/21187662/
4. Yamaoka, Mami, Ishizaki, Toshimasa, Kimura, Toshihide. . Interplay between Rab27a effectors in pancreatic β-cells. In World journal of diabetes, 6, 508-16. doi:10.4239/wjd.v6.i3.508. https://pubmed.ncbi.nlm.nih.gov/25897360/
5. Li, Qingyan, Zhao, Huixia, Dong, Weiwei, Yang, Jingwen, Xiao, Wenhua. 2022. RAB27A promotes the proliferation and invasion of colorectal cancer cells. In Scientific reports, 12, 19359. doi:10.1038/s41598-022-23696-7. https://pubmed.ncbi.nlm.nih.gov/36371494/
6. Ma, Xiaotang, Zhao, Jia, Li, Suqing, Chen, Yanfang, Pan, Qunwen. 2022. Rab27a-dependent exosomes protect against cerebral ischemic injury by reducing endothelial oxidative stress and apoptosis. In CNS neuroscience & therapeutics, 28, 1596-1612. doi:10.1111/cns.13902. https://pubmed.ncbi.nlm.nih.gov/35770324/
7. Kimura, Toshihide, Niki, Ichiro. 2011. Rab27a in pancreatic beta-cells, a busy protein in membrane trafficking. In Progress in biophysics and molecular biology, 107, 219-23. doi:10.1016/j.pbiomolbio.2011.06.016. https://pubmed.ncbi.nlm.nih.gov/21762718/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen